| 鸡传染性贫血病毒与禽网状内皮增生症病毒二重PCR检测方法的建立 |
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| 引用本文： | 吴萍萍,张小飞,潘玲,胡来根,尹秀凤,毛火云.鸡传染性贫血病毒与禽网状内皮增生症病毒二重PCR检测方法的建立[J].中国畜牧兽医,2010,37(4):182-184. |
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| 作者姓名： | 吴萍萍 张小飞 潘玲 胡来根 尹秀凤 毛火云 |
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| 作者单位： | （1.安徽农业大学动物科技学院， 合肥 230036； 2.江苏省农业科学院， 南京 210014；3.南京天邦生物科技有限公司， 南京 211102） |
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| 摘 要： | 当前养鸡业中鸡传染性贫血病毒、禽网状内皮增生症病毒混合感染的发生率日益增高，为提高CIAV、REV检测效率，根据GenBank上登录的CIAV全基因序列和REV的LTR序列分别设计了2对引物，建立了REV、CIAV二重PCR检测方法，另外还比较了2种不同的DNA提取方法。结果显示，该方法扩增目的条带清晰、敏感性高、特异性好。应用建立的方法对临床7份病料样品检测，检出5份CIAV、REV阳性，表明建立的二重PCR方法能有效用于CIAV、REV混合感染的临床诊断。
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| 关 键 词： | 鸡传染性贫血病毒(CIAV) 禽网状内皮增生症病毒(REV) 二重PCR |
Establishment of a Double PCR Assay for Detection of Chicken Anaemia Virus and Reticuloendotheliosis Virus |
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| WU Ping-ping,ZHANG Xiao-fei,PAN Ling,HU Lai-gen,YIN Xiu-feng,MAO Huo-yun.Establishment of a Double PCR Assay for Detection of Chicken Anaemia Virus and Reticuloendotheliosis Virus[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):182-184. |
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| Authors: | WU Ping-ping ZHANG Xiao-fei PAN Ling HU Lai-gen YIN Xiu-feng MAO Huo-yun |
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| Institution: | (1.College of Animal Science and Technology，Anhui Agricultural University，Hefei 230036，China； 2.Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China； 3.Nanjing Tech-bank Bio-industry Co.LTD，Nanjing 211102，China) |
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| Abstract: | The co-infection incidence of chicken anaemia virus(CIAV),reticuloendotheliosis virus(REV) are increasing in present poultry industry,in order to enhance the detection efficiency of CIAV and REV,according to the sequence of whole gene of CIAV and the LTR of REV published in GenBank,two pairs of primers were designed,the double PCR of REV,CIAV was established.Moreover,two kinds of extraction method of DNA were compared.The results showed that the object fragments were amplified and hold high sensitivity and ... |
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| Keywords: | CIAV REV double PCR detection co-infection |
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