Axenic culture and influence of wetness period and inoculum concentration on infection and development of cercospora blight ofHeliotropium europaeum |
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Authors: | S. Hasan M. Jourdan L. Brun A. W. Sheppard |
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Affiliation: | (1) CSIRO Biological Control Unit, Campus International de Baillarguet, 34982 Montferrier-sur-Lez Cedex, France;(2) CSIRO Division of Entomology, GPO Box 1700, 2601 Canberra, ACT, Australia |
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Abstract: | Cercospora heliotropii-bocconii is a fungal pathogen of the ephemeral annual weedHeliotropium europaeum. The effects of wetness period and inoculum concentration on disease severity were studied under controlled conditions. The fungus was grown on different artificial culture media and carrot juice agar with 5 g l–1 yeast extract was found to be the most suitable medium for conidial production under artificial conditions. Abundant disease symptoms only occurred after 8 h of wetness at 20°C. The minimum incubation period before disease symptoms appeared was 8 days following a wetness period of at least 40 h. Inoculum concentration of 1×104 conidia per ml killed plants in less than one month and reduced seed production by two thirds. These results suggest that this pathogen has the potential to reduce plant survival and seed bank replenishment of this annual weed species. |
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Keywords: | biological control of weeds biology Cercospora heliotropii-bocconii common heliotrope Heliotropium europaeum wetness period |
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