| 黑龙江省水稻品种(系)孕穗期耐冷性SSR标记的筛选 |
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| 引用本文： | 王麒,冯延江,曾宪楠,张小明,卞景阳. 黑龙江省水稻品种(系)孕穗期耐冷性SSR标记的筛选[J]. 东北农业大学学报, 2010, 41(3) |
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| 作者姓名： | 王麒 冯延江 曾宪楠 张小明 卞景阳 |
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| 作者单位： | 黑龙江省农业科学院耕作栽培研究所,哈尔滨,150086;东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省农业科技创新工程重点项目 |
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| 摘 要： | 研究通过对黑龙江省不同地区的120份水稻品种(系)进行孕穗期耐冷性鉴定,明确不同水稻品种(系)的孕穗期耐冷性差异。根据BSA方法,借助SSR分子标记手段,挑选孕穗期耐冷最强和最弱的极端值各12个DNA样本等量混合,组成抗感两个池,利用国内外已发表的一些与孕穗期耐冷性主效QTL相连锁的SSR标记一同筛选。通过相关性分析和符合度计算,得到位于水稻第11染色体和第1染色体上RM229、RM259与黑龙江水稻孕穗期耐冷性通用性高的引物,两个引物与黑龙江水稻孕穗期耐冷性抗感池品种的相似性系数为0.75和0.58,准确率达83.3%和75%,可用于黑龙江水稻品种(系)资源和相应后代孕穗期耐冷性的筛选。
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| 关 键 词： | 水稻品种(系) 孕穗期 耐冷性 SSR 筛选 |
Screening of rice varieties (lines) cold tolerance used SSR marker at booting stage in Heilongjiang Province |
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| WANG Qi,FENG Yanjiang,ZENG Xiannan,ZHANG Xiaoming,BIAN Jingyang. Screening of rice varieties (lines) cold tolerance used SSR marker at booting stage in Heilongjiang Province[J]. Journal of Northeast Agricultural University, 2010, 41(3) |
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| Authors: | WANG Qi FENG Yanjiang ZENG Xiannan ZHANG Xiaoming BIAN Jingyang |
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| Affiliation: | 1.Crop Tillage and Cultivation Institute;Heilongjiang Academy of Agricultural Sciences;Harbin 150086;China;2.College of Agriculture;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | This paper studied cold tolerance of 120 rice varieties(lines) planted in different regions of Heilongjiang Province at booting stage,cleared the differences of rice varieties(lines) cold tolerance.According to BSA method,selected each 12 DNA samples mixed equally by SSR markers at booting stage,which of cold tolerance of extreme values were the strongest and the weakest,composited resistant and susceptible pools,The screening of the main effect QTL linked with cold tolerance at booting stage SSR markers th... |
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| Keywords: | SSR rice varieties (lines) booting stage cold tolerance SSR screening |
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