Sampling of Sugarcane Leaves in Field Experiments to Determine the Activity of Nitrate Reductase |
| |
| Authors: | Renato Lemos dos Santos Emídio Cantídio Almeida de Oliveira José de Arruda Barbosa Maria José Alves de Moura Nayara Rose da Conceição Lopes |
| |
| Affiliation: | 1. Educational Development Department, Federal Institute of Education, Science and Technology, Campus Vitória de Santo Ant?o, Pernambuco, Brazil;2. Agrnomy Department, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil. |
| |
| Abstract: | Nitrogen (N) assimilation depends on nitrate reductase activity (NRA). Leaf sampling methods in field experimentation with sugarcane and analytical procedures are essential for the determination of the maximum activity (MA) of the enzyme. Thus, in five tests, it was possible to determine which period of the crop cycle should be used for leaf collection, the most adequate leaf for collection, the ideal number of leaves to be collected, the period of the day in which the enzyme has MA and which analytical procedures can be optimized. The evaluation of NRA in sugarcane should occur between 95 and 100 days after planting (DAP) and can be performed in any green leaf. In field experiments, it is recommended the collection of 15 leaves, which must occur between 12:00 and 12:30 h, with later incubation of the leaf tissues for 430 min, without vacuum application. |
| |
| Keywords: | Diagnostic leaves enzyme activity leave sampling nitrogen absorption nitrogen assimilation |
|
|
