| 小麦淀粉GBSSII基因的克隆、反义和RNAi载体的构建 |
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| 引用本文： | 康国章 肖向红 王永华 郭天财 朱云集 官春云. 小麦淀粉GBSSII基因的克隆、反义和RNAi载体的构建[J]. 华北农学报, 2008, 23(1): 7-11 |
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| 作者姓名： | 康国章 肖向红 王永华 郭天财 朱云集 官春云 |
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| 作者单位： | 河南农业大学，河南农业大学，河南农业大学，河南农业大学，河南农业大学，河南农业大学 河南郑州450002，河南郑州450002，河南郑州450002，河南郑州450002，河南郑州450002，河南郑州450002，湖南农业大学，湖南长沙461012 |
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| 基金项目： | 中国博士后科学基金(20060390773)，河南省教育厅自然科学基金(2006210007) |
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| 摘 要： | 采用RT-PCR方法从小麦品种豫教2号籽粒中克隆出颗粒型淀粉合成酶II基因(Granule bound starch syn-thase,GBSSII)部分cDNA片段(1 069 bp)(GenBank登录号:EF221764),同源性比较结果显示,它与GenBank上已报道的GBSSII基因有高度的同源性。以pWM101质粒为基础,构建了由35 S启动子调控的GBSSII基因的反义表达载体pWM101GBSSIIA;另外,还以pFGC5941质粒为基础,构建了GBSSII基因的RNAi载体pFGC5941GBSSIIsa,这些载体的构建为研究GBSSII基因的功能打下了很好的基础。
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| 关 键 词： | 普通小麦 淀粉合成酶II基因 表达载体 |
| 文章编号： | 1000-7091（2008）01-0007-05 |
| 收稿时间： | 2007-09-03 |
| 修稿时间： | 2007-09-03 |
Molecular Cloning of Granule Bound Starch Synthase |
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| KANG Guo-zhang ,XIAO Xiang-hong ,WANG Yong-hua, GUO Tian-cai ,ZHU Yun-ji ,GUAN Chun, yun. Molecular Cloning of Granule Bound Starch Synthase[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(1): 7-11 |
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| Authors: | KANG Guo-zhang XIAO Xiang-hong WANG Yong-hua GUO Tian-cai ZHU Yun-ji GUAN Chun yun |
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| Abstract: | Partial cDNA sequences(1 069 bp)of granule bound starch synthase II(GBSSII)gene(GenBank No.EF221761)was amplified from grains of common wheat cultuvar Yujiao 2 by RT-PCR in the paper.The result demonstrated that the cloned GBSSII gene sequence had 95%-97% identity with the reported GBSSII genes in GenBank.In addition,its antisense expression(pWM101GBSSIIA)vector was constructed with pMW101 vector,and RNAi vector(pFGC5941GBSSIIsa)was also constructed with pFGC5941.These constructed vectors would provide a good background for investigting the function of GBSSII on biosynthesis of starch in wheat plants. |
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| Keywords: | Common wheat(Triticum aestivum) GBSSII Expression vecors |
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