| 金鱼核糖体转录间隔区(ITS-1)的克隆和序列分析 |
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| 引用本文： | 牟希东,白俊杰,汪学杰,罗建仁.金鱼核糖体转录间隔区(ITS-1)的克隆和序列分析[J].淡水渔业,2008,38(1):74-76,43. |
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| 作者姓名： | 牟希东 白俊杰 汪学杰 罗建仁 |
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| 作者单位： | 1. 中国水产科学院热带亚热带鱼类选育与养殖重点开放实验室,中国水产科学研究院珠江水产研究所,广州,510380;上海水产大学,上海,200090
2. 中国水产科学院热带亚热带鱼类选育与养殖重点开放实验室,中国水产科学研究院珠江水产研究所,广州,510380 |
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| 摘 要： | 对金鱼(Carassius auratus)核糖体转录间隔区(ITS-1)进行了克隆测序,并对ITS-1序列进行了分析。结果显示,克隆的金鱼ITS-1区序列长度为294bp,其中A、T、G、C 4种碱基的含量分别为17.0%、14.6%、33.0%、35.4%;与从GenBank中检索到的12种鱼的核糖体DNA序列比较显示,其与12种鱼的ITS-1序列同源性较低,在26.0%~61.6%之间;根据金鱼与其他12种鱼的ITS-1序列的同源性构建进化树,所得的分类结果与传统的分类结果基本一致。
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| 关 键 词： | 金鱼(Carassius auratus) 核糖体 转录间隔区1(ITS-1) 基因克隆 基因序列 |
| 文章编号： | 1000-6907-（2008）01-0074-03 |
| 收稿时间： | 2007-09-07 |
| 修稿时间： | 2007年9月7日 |
Cloning and Sequence Analysis of Ribosomal DNA Internal Transcribed Spacer 1 From Goldfish(Carassius auratus) |
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| MU Xi-dong,BAI Jun-jie,WANG Xue-jie,LUO Jian-ren.Cloning and Sequence Analysis of Ribosomal DNA Internal Transcribed Spacer 1 From Goldfish(Carassius auratus)[J].Freshwater Fisheries,2008,38(1):74-76,43. |
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| Authors: | MU Xi-dong BAI Jun-jie WANG Xue-jie LUO Jian-ren |
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| Abstract: | The internal transcribed spacer 1(IST-1) from goldfish(Carassius auratus) was cloned,sequenced and analyzed.The result showed a sequence with total length of 294 nucleotides of ITS1.The contents of A,T,G and C were 17.0%,14.6%,33.0%,35.4% in ITS-1 of goldfish.Comparing to ITS-1 sequences of other 12 fishes in GenBank,there were lower homologies from 26.0% to 61.6% between goldfish and other fishes.The evolution tree built from the ITS-1 homology of goldfish and other 12 fishes was consistent with that of traditional taxonomic results. |
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| Keywords: | goldfish(Carassius auratus) ribosomal DNA internal transcribed spacer 1(IST-1) gene cloning DNA sequencing |
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