水稻农杆菌转化体系中共培养方法的优化

目的]对水稻农杆菌转化体系中共培养过程进行优化。【方法】以水稻品种日本睛作为材料，对2种不同的共培养方法，即将1层无菌滤纸铺于固体共培养基表面和用0．5mLAAM培养液浸湿的无菌滤纸的方法进行比较。【结果】无菌滤纸用0．5mLAAM培养液浸湿后铺于培养皿，后直接将浸染后的愈伤组织在其上进行共培养，效果好且染菌少。【结论】利用0．5mLAAM浸湿的滤纸培养既简化操作又有利于愈伤组织生长。

Optimization on Co-culture of Agrobacterium-mediated Transformation in Oryza sativa

Objective] The co-culture of Agrobacterium-mediated transformation in Oryza sativa was optimized. Method] With Oryza sativa L.japonica. cv. Nipponbare as the tested material, took two different co-culture methods were set up to study the effect of 0.5 mL AAM on co-culture. Result] The effect of filter paper soaked with 0.5 mL AAM was good and infected bacteria less. Conclusion] The filter paper soaked with 0.5 mL AAM was beneficial to the callus formation.