鹅源性成分PCR检测方法的建立

[目的]建立一种检测鹅源性成分的PCR方法。[方法]以15种动物的DNA为模板，利用鹅的特异性引物进行PCR反应，PCR产物用1．4％的琼脂糖凝胶电泳检测并测序，检测引物的特异性和敏感性。[结果]通过PCR反应从鹅样品中扩增到了304bp的目的片段，其他物种火鸡、鸭、鸽鹑、鸵鸟、鸽子、鸡、马、牛、羊、猪、狗、驴、猫、鱼和空白对照则无目的片段，表明该引物的特异性良好。鹅组织PCR产物的核苷酸序列与GenBank中检索到的相应序列基本符合，相似性为98％；PCR检测方法的检测灵敏度为0．01％。[结论]该PCR检测方法特异性强、准确性好、灵敏度高、可重复性好，为明确食品与饲料的成分和来源，预防禽流感的传播提供了有效的分子生物学捡测方法.

Establishment of PCR Detections for Goose-derived Components

[Objective] The purpose was to establish a PCR method for detecting goose-derived components.[Method] With DNAs from 15 animals as templates,PCR reactions were carried out by using specific primers of goose.The PCR products were detected by 1.4% agarose gel electrophoresis and sequenced to detect the specificity and sensitivity of primers.[Result] The target fragment with length as 304 bp was amplified from goose samples through PCR reaction.There was no target fragment in other species such as turkeys,duck...