| 石油污染土壤中芳烃降解菌及邻苯二酚2,3双加氧酶的克隆 |
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| 引用本文： | 吴宇澄,骆永明,滕应,刘五星,李振高. 石油污染土壤中芳烃降解菌及邻苯二酚2,3双加氧酶的克隆[J]. 土壤, 2006, 38(5): 640-644 |
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| 作者姓名： | 吴宇澄 骆永明 滕应 刘五星 李振高 |
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| 作者单位： | 1. 中国科学院南京土壤研究所土壤与环境生物修复研究中心,南京,210008;土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008;中国科学院研究生院,北京,100049
2. 中国科学院南京土壤研究所土壤与环境生物修复研究中心,南京,210008;土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008 |
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| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划);中国科学院知识创新工程项目 |
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| 摘 要： | 石油污染土壤中的芳烃降解菌是进行土壤修复的主要生物资源,本研究对某炼油厂附近土壤中的芳烃降解菌及邻苯二酚2,3双加氧酶基因进行了研究。结果表明,部分石油烃污染土壤中存在着大量的芳烃降解菌;对其中一个土壤样本中的邻苯二酚2,3双加氧酶基因进行克隆,获得了7个不同的邻苯二酚双加氧酶基因序列,序列分析表明这些基因可能来源于土壤中的假单胞菌,且该基因在土壤中的丰度与污染水平及芳烃降解菌的数量相关。可见,土壤中芳烃降解菌数量及降解基因的丰度和多样性,可以对石油污染土壤的生物修复进行监控并为生物修复提供丰富的微生物资源。
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| 关 键 词： | 石油污染 土壤 邻苯二酚2 3双加氧酶 芳烃降解细菌 |
Aromatic Hydrocarbons Degraders in Oil-contaminated Soils and Cloning of Catechol 2,3-Dioxygenase Gene |
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| WU Yu-cheng,LUO Yong-ming,TENG Ying,LIU Wu-xin and LI Zhen-gao. Aromatic Hydrocarbons Degraders in Oil-contaminated Soils and Cloning of Catechol 2,3-Dioxygenase Gene[J]. Soils, 2006, 38(5): 640-644 |
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| Authors: | WU Yu-cheng LUO Yong-ming TENG Ying LIU Wu-xin LI Zhen-gao |
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| Affiliation: | Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China;Graduate School of the Chinese Academy of Sciences, Beijing 100049, China,Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China;Graduate School of the Chinese Academy of Sciences, Beijing 100049, China,Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China,Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China;Graduate School of the Chinese Academy of Sciences, Beijing 100049, China and Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China |
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| Abstract: | Indigenous aromatic hydrocarbons degraders (AHD) are the primary member for bioremediation of oil-contaminated soils. In the present study, the aromatic hydrocarbons degraders and catechol 2,3-dioxygenase (C23O) gene were determined. Abundant degradative bacteria were detected and a 934 bp DNA fragment of C23O was amplified in two of four heavily contaminated soils (oil content>10000 mg/kg), indicating a relationship between abundance of AHD or C23O copies and oil content. Sequences analysis of C23O gene implied a possible pseudomonad origin. This study suggested a possibility of monitoring bioremediation of oil-contaminated soils and screening degradative bacteria resources by determining the number of aromatic hydrocarbons and abundance or diversity of degradative genes in soil. |
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| Keywords: | Oil contamination Soil Catechol 2 3-dioxygenase Aromatic hydrocarbons degraders |
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