快大型肉用种鸡遗传变异的RAPD分析

以RAPD技术对AA、科宝和艾维茵3种肉鸡的父母代父本和母本共6个群体用28个引物进行遗传变异分析结果表明,6个群体平均相似指数为70.7％,艾维茵鸡母本与科宝鸡母本间相似指数最大达81.0％;艾维茵母本与AA鸡父本间相似系数最小为61.2％。品种内相似指数不完全大于品种间相似指数,艾维茵母本与AA鸡父本配套可望获得较好的杂种优势。DNA标记技术为探讨外来鸡种的遗传资料提供了手段,对引进品种的选择和如何配套生产有重要的参考价值。

The genetic variation analysis of the fast-growing broiler breeders by RAPD marking technique

RAPD (Random Amplified Polymorphic DNA) was used with 28 primers to analyze the genetic variation among the sires and the dams of AA,Avain,and Cobb chickens in this experiment.The results show that the average similarity coefficient of the 6 groups is 70.7%,the dams of Avain and Cobb chicken share the highest similarity coefficient at 81.0 %,and Avf and AAm share the lowest similarity coefficient at 61.2 %.And the similarity coefficient within the breed is not necessary greater than that of breed to breed,showing that the correctness of the present broiler breeding program,and implying that the crossbreeding of Avf and AAm will attain the maximum heterosis.DNA marking technology provides methods for studying the genetic materials of foreign breed chickens,and it is also the important references for selecting broiler breeds and crossbreeding.