Tissue culture ofRosa Chinensis var.Floribunda |
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Authors: | Zhu Hong |
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Institution: | (1) Northeast Forestry University, 150040 Harbin, China |
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Abstract: | The tissue culture of Schloss Mannheim(Rosa Chinensis var.Flaribunda) with full and unsprouting bud of stem segments as the explants was experimented. The result shows that the buds sprouted
best on MS medium with the addition of 6-BA 1.0 mg/L, and differentiation was best on MS medium with addition of 6-BA 1.5
+ NAA 0.05 + ZT 0.1 mg/L or KT 1.0 + NAA0.05 + ZT 0.1 mg/L. The MS medium with addition of 6-BA 0.3 + NAA 0.0 5+ ZT 0.1 mg/L
or KT 0.3 + NAA0.05 + ZT 0.1 mg/L showed a good result for developing strong shoots. 1/2 MS medium with the addition of IBA
0.1 mg/L or IBA 0.1 + NAA 0.02 mg/L had best result for rooting. The plantlets should be transplanted from test-tube to soil
when they grew to 2.5 ∼ 4.0 cm high and have 3 ∼ 5 strips short roots. A higher survival rate was obtained under the conditions
of controlling humidity and temperature. |
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Keywords: | Rosa Chinensis var Floribund Tissue culture Cvtokinin Auxin |
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