RAPD markers linked to a clubroot-resistance locus in Brassica rapa L. |
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Authors: | Yasuhisa Kuginuki Hidetoshi Ajisaka Mamiko Yui Hiroaki Yoshikawa Ken-ichi Hida Masashi Hirai |
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Institution: | (1) Ornamental Plants and Tea (NIVOT), National Research Institute of Vegetables, Ano, Mie 514-23, Japan |
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Abstract: | Linkage of random amplified polymorphic DNA (RAPD) markers with resistance genes to clubroot (Plasmodiophora brassicae Wor.)
in Brassica rapa L. was studied in a doubled haploid (DH population obtained by microspore culture. Thirty-six DH lines were
obtained from F1 plants from a cross between susceptible ‘Homei P09’ and resistant ‘Siloga S2’ plants. ‘Homei P09’ was a DH line obtained
by microspore culture of the Chinese cabbage variety ‘Homei’, which is highly responsive in microspore culture. The resistant
line ‘Siloga S2’ was obtained by two rounds of selfing of the fodder turnip ‘Siloga’. Three RAPD markers, RA12-75A, WE22B
and WE49B, were found to be linked to a clubroot-resistance locus. These three markers were linked in the DH lines and an
F2 population and should be useful for marker-assisted selection in breeding programs.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Chinese cabbage doubled haploid microspore culture Plasmodiophora brassicae Random Amplified Polymorphic DNA |
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