枸杞叶片再生植株体系的建立

以"宁杞2号"为试材,研究了激素浓度、叶片生理状态和光照条件等因素对叶片再生的影响,建立了枸杞叶片外植体的不定芽高频再生体系。研究结果表明:以顶部充分伸展的35d叶龄的无菌苗叶片为外植体,再生效果好。将此类叶片放在含1 0mg L6-BA和1 0mg LIBA的1 2MS分化培养基上,暗培养8d后转到光下培养,28d后可见到不定芽不经过或经过很少的愈伤组织阶段,直接从叶片上分化产生,出现的高峰期在接种后3545d,芽分化率高达90 0%以上。待小芽长至1～2cm后将其从叶片上剪下,转到生根培养基(1 2MS+0 6mg LNAA+0 2mg LIBA)上得到完整植株。该再生体系可作为基因转化的受体系统。

Plant regeneration from leaf in Lycium barbarum

In this paper, the "No.2" of Lycium barbarum leaf regeneration system was thoroughly discussed. The leaves of "No.2" of Lycium barbarum were used as the experimental materials. Several factors such as hormone concentration, explant condition illumination condition and so on were investigated to optimize the regeneration system in vitro. The experimental results showed that leaf situation could greatly affect "No.2" of Lycium barbarum regeneration frequency. The best explants were 35 days apical leaves from the cultural plantlets. The high frequency of shoot regeneration was observed when leaf explants were cultured on 1/2 MS medium supplemented with 1.0 mg/L 6-BA and 1.0 mg/L IBA. Firstly, the leaves were cultured in dark. After 8 days, the explants were transferred under light and began to directly regenerate adventitious buds in about 28 days. The maximum number of the adventitious buds were observed within 35 to 45 days. The shoot differentiation frequency was more than 90.0%. When the shoots grow to 1～2 cm high, they were cut from leaves, then transferred on 1/2 MS+0.6 mg/L NAA+0.2 mg/L IBA and developed into whole plants. This regeneration system could applied to Lycium barbarum transgenic manipulation.