| 猪心中提取和纯化辅酶Q10（联产CytC） |
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| 引用本文： | 袁艺.猪心中提取和纯化辅酶Q10（联产CytC）[J].安徽农业大学学报,1997,24(2):200-203. |
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| 作者姓名： | 袁艺 |
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| 作者单位： | 安徽农业大学生物工程系 |
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| 摘 要： | 辅酶Ｑ１０和细胞以素Ｃ是促生物氧化酶类生化药物，本试验从猪心中提了和纯化辅酶Ｑ１０，并联产细胞以素Ｃ。猪心以酸性水液粗提，细胞色素Ｃ经人造沸石吸附，洗脱，盐析，三氯乙酸沉淀，弱酸性阳离子吸附，真空干燥得２２５ｍｇＣｙｔＣ／ｋｇ猪心，纯度７９％，提取后的猪心渣，用醇皂化法，经硅胶桂层板，无水乙醇结晶，重结晶，每千克猪心中撮辅酶Ｑ１０７５ｍｇ，含量为９５．３％。
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| 关 键 词： | 辅酶Q10 细胞色素C 化学提取 纯化 猪心 心脏 |
Extraction and Purification of Coenzyme Q 10 and Cytochrome C from Pig Heart |
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| Yuan Yi.Extraction and Purification of Coenzyme Q 10 and Cytochrome C from Pig Heart[J].Journal of Anhui Agricultural University,1997,24(2):200-203. |
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| Authors: | Yuan Yi |
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| Abstract: | Coenzyme Q 10 and Cytochrome C are biochemical medicine of biooxidase.The artical has developed a method of extracting and purification of coenzyme Q 10 and cytochrome C from pig heart.Pig heart is ronghly treated in secondary acid liquid and 225 mg cytochrome C (purity 79%) from 1 kg pig heart is obtained through adsorption,eluting,salting out,precipitating,vacuum drying.75 mg coenzyme (Q 10 concentration 95 3%) is extracted from 1 kg pig heart residue through saponification,chromatography and crytallization. |
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| Keywords: | Coenzyme Q 10 Cytochrome C |
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