栀子茎尖培养基法快速繁育研究

目的]通过对栀子茎尖进行组织培养和快速繁殖试验,为栀子的产业化生产提供参考和依据。方法]以栀子茎尖为外植体,通过诱导分化,增殖,生根等组织培养法来快速繁殖栀子幼苗,最后对试管幼苗进行驯化移栽。结果]MS＋BA 1.2 mg/L＋NAA 0.1 mg/L的培养基有利于栀子芽的萌发,成活率高达100% MS＋BA 2.0 mg/L＋NAA 0.1 mg/L的培养基有利于增殖 1/2 MS＋NAA 0.1 mg/L的培养基生根效果好 在比例按m（珍珠岩）∶m（草木灰）∶m（菜园土）=1∶1∶1混合并经过消毒处理的基质中移栽幼苗成活率高。结论]以栀子茎尖为外植体,采用组织培养技术可以实现栀子幼苗的快速繁殖。

Shoot Tip Tissue Culture and Rapid Propagation of Gardenia

Objective] The aim of this research was to study the shoot tip tissue culture and rapid propagation,and provide reference basis for the industrialization production of gardenia.Method] With shoot tip of gardenia as explants, gardenia seedlings were abtained from tissue culture such as induction,differentiation,rooting and then transplantated and domesticated.Result] The MS+BA 1.2 mg/L+NAA 0.1 mg/L culture medium is advanta-geous to the germinaton of gardenia buds and the survival rate reached 100%.The cu...