| 大田软海绵酸对HL-7702肝细胞毒性效应的研究 |
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| 引用本文： | 黄爱君,黄海燕,庄志雄,杨汝德,刘建军. 大田软海绵酸对HL-7702肝细胞毒性效应的研究[J]. 安徽农业科学, 2010, 38(10): 5251-5253. DOI: 10.3969/j.issn.0517-6611.2010.10.070 |
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| 作者姓名： | 黄爱君 黄海燕 庄志雄 杨汝德 刘建军 |
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| 作者单位： | 华南理工大学生物科学与工程学院,广东,广州,510006;深圳市疾病预防控制中心,深圳市现代毒理学重点实验室,广东,深圳,518020;深圳市疾病预防控制中心,深圳市现代毒理学重点实验室,广东,深圳,518020;华南理工大学生物科学与工程学院,广东,广州,510006 |
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| 基金项目： | 深圳市科技计划资助项目 |
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| 摘 要： | [目的]探讨大田软海绵酸（okadaic acid,OA）对HL-7702肝细胞的毒性效应。[方法]通过细胞计数试剂盒（Cell Counting Kit-8,CCK-8）、细胞形态学观察及流式细胞术等方法,从细胞增殖、细胞凋亡等方面探讨OA对HL-7702肝细胞的毒性效应。[结果]与溶剂对照组相比,OA对HL-7702肝细胞增殖有明显的抑制作用,细胞增殖抑制率随着OA浓度的增加而增加,同时随着染毒时间的延长,细胞增殖抑制率也明显增加;与对照组相比,作用HL-7702肝细胞24h后,5nmol/LOA可明显诱导细胞发生凋亡。[结论]OA可以通过抑制细胞增殖及诱导细胞凋亡的方式对HL-7702肝细胞产生毒性效应,且呈现剂量-效应关系。
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| 关 键 词： | 大田软海绵酸 细胞增殖 凋亡 HL-7702肝细胞 |
Study on the Cytotoxic Effect of Okadaic Acid on HL-7702 Liver Cells |
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| Affiliation: | HUANG Ai-jun et al (College of Bioscience and Engineering,South China University of Technology,Guangzhou,Guangdong 510006) |
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| Abstract: | [Objective] The research aimed to study the cytotoxicity of okadaic acid on HL-7702. [Method] The toxic effect of OA on HL-7702 liver cells was investigated from the aspects of cell proliferation and apoptosis by using Cell Counting Kit-8, cell morphology observation and flow cytometry. [Result] Compared with the control group, OA inhibited the proliferation of HL-7702 liver cells significantly, and the inhibition ratio of cell proliferation increased along with the increase of OA concentration and exposure time. Compared with the control group, 5 nmol/L OA could induce apoptotic cells significantly. [Conclusion] OA exhibited a dose-dependent cytotoxic effect on HL-7702 liver cells by the inhibition of cell proliferation and induction of apoptosis. |
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| Keywords: | Okadaic acid Cell proliferation Apoptosis HL-7702 liver cells |
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