携带四环素耐药基因的鸭源大肠杆菌质粒不相容群分析

目的:用PCR方法测定22株携带四环素耐药基因鸭大肠杆菌所含质粒的不相容群。方法:设计18对引物,分为5个多重PCR体系和3个单一PCR体系,用来检测识别FIA、FIB、FIC、HI1、HI2、I1、L/M、N、P、W、T、A/C、K、B/O、X、Y、F和FⅡ复制子。结果:22株鸭大肠杆菌中,分别有7、4、9、2、6、2、3、7株和19株属于IncI1、IncN、IncFIB、IncY、IncP、IncFIC、IncA/C、In-cK和IncFⅡ。试验菌株所含质粒大部分属于IncFⅡ,占86.4%,属于1、2、3、4、5个不相容群组合的分别为13.6%、31.8%、31.8%、18.2%和4.5%。

Analysis of Incompatible Groups of Plasmids in Duck-derived Escherichia coli Carrying Tetracycline Resistant Genes

Objective: The incompatible groups of the plasmids carrying tetracycline resistant genes in 22 duck Escherichia coli isolates were determined by using PCR method.Methods: 18 pairs of designed primers were divided into five multiple PCR systems and three single PCR systems,and they were used to detect FIA,FIB,FIC,HI1,HI2,I1,L/M,N,P,W,T,A/C,K,B/O,X,Y,F and FⅡ replicons.Results: seven,four,nine,two,six,two,three,seven and nineteen of 22 E.coli isolates from duck belonged to IncI1,IncN,IncFIB,IncY,IncP,IncFIC,IncA/C,IncK and IncFⅡ,respectively.Most(86.4%) of plasmids in the tested strains belonged to IncFII.One incompatible group and 2,3,4 and 5 incompatible group combinations accounted for 13.6%,31.8%,31.8%,18.2% and 4.5%,respectively.