| 矿物元素和葡萄糖对木聚糖酶活力影响的研究 |
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| 引用本文： | 李淑丽,王娇,尹清强,常娟,左瑞雨,郑秋红,王平,王潇,刘俊熙.矿物元素和葡萄糖对木聚糖酶活力影响的研究[J].江西农业学报,2012,24(1):106-108,111. |
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| 作者姓名： | 李淑丽 王娇 尹清强 常娟 左瑞雨 郑秋红 王平 王潇 刘俊熙 |
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| 作者单位： | 1. 河南农业大学牧医工程学院,河南郑州,450002
2. 河南省饲料微生物工程技术研究中心,河南周口,466000 |
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| 基金项目： | 河南省郑州市科技创新团队项目 |
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| 摘 要： | 为探讨硫酸铵、氯化钙、磷酸氢二钾、植酸钙镁和葡萄糖对米曲霉产木聚糖酶能力的影响,采用5因素3水平的正交设计方法对其进行了分析。结果表明:矿物元素和葡萄糖对木聚糖酶活力均有一定的影响,其影响顺序为:硫酸铵>磷酸氢二钾>葡萄糖>植酸钙镁>氯化钙,最优组合的添加比例分别为1.00%、0.40%、0.00%、0.00%、0.00%。其最高酶活出现在固体培养的72 h,木聚糖酶活力达到537.56 U/g(P<0.05)。
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| 关 键 词： | 米曲霉 木聚糖酶 矿物元素 葡萄糖 |
Study on Effects of Mineral Elements and Glucose on Xylanase Activity |
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| LI Shu-li
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WANG Jiao
,
YIN Qing-qiang
,
CHANGJuan
,
ZUO Rui-yu
,
ZHENG Qiu-hong
,
WANG Ping
,
WANG Xiao
,
LIU Jun-xi.Study on Effects of Mineral Elements and Glucose on Xylanase Activity[J].Acta Agriculturae Jiangxi,2012,24(1):106-108,111. |
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| Authors: | LI Shu-li WANG Jiao YIN Qing-qiang CHANGJuan ZUO Rui-yu ZHENG Qiu-hong WANG Ping WANG Xiao LIU Jun-xi |
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| Institution: | 1.College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Provincial Research Center of Feed Microbe Engineering & Technology,Zhoukou 466000,China) |
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| Abstract: | To determine the effects of ammonium sulfate,calcium chloride,dipotassium hydrogen phosphate,phytin and glucose on xylanase production of Aspergillus oryzae,the orthogonal design(5 factors× 3 levels) was used in this study.The results showed that some mineral elements and glucose could affect xylanase production,the order of their influence was ammonium sulfate> dipotassium hydrogen phosphate> glucose> phytin> calcium chloride,and their optimum addition rates were 1.00%,0.40%,0.00%,0.00% and 0.00%,respectively.The highest xylanase activity was 537.56 U/g at the 72nd hour after solid-state fermentation(P<0.05). |
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| Keywords: | Aspergillus oryzae Xylanase Mineral elements Glucose |
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