| 金叶山梅花的组织培养研究 |
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| 引用本文： | 林颖,顾德峰.金叶山梅花的组织培养研究[J].安徽农业科学,2010,38(32):18048-18049,18106. |
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| 作者姓名： | 林颖 顾德峰 |
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| 作者单位： | 吉林农业大学园艺学院,吉林长春,130118;吉林农业大学园艺学院,吉林长春,130118 |
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| 基金项目： | 吉林省重大科技项目(20060222) |
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| 摘 要： | 目的]加快金叶山梅花繁殖速度,扩大其应用。方法]以金叶山梅花的茎尖及带腋芽的幼嫩茎段为外植体,进行组织培养,以找出最适合其生长的培养基。结果]金叶山梅花的启动培养基为MS＋0.05 mg/L NAA＋0.50 mg/L 6-BA,启动率达100.0%;最佳增殖培养基为MS＋0.05 mg/L NAA＋0.50 mg/L 6-BA,增殖系数为3.40;以1/2MS＋0.50 mg/L NAA作为生根培养基,生根率可达100.0%。上述条件培养得到的幼苗,移栽时以草炭土为基质,成活率达92%以上。结论]填补了我国对金叶山梅花快繁生产研究的空白,为其工厂化生产提供了参考。
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| 关 键 词： | 金叶山梅花 茎尖 茎段 组织培养 |
Studies on the Tissue Culture of Philadelphus coronarius 'Aureus' |
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| LIN Ying et al.Studies on the Tissue Culture of Philadelphus coronarius 'Aureus'[J].Journal of Anhui Agricultural Sciences,2010,38(32):18048-18049,18106. |
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| Authors: | LIN Ying |
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| Institution: | LIN Ying et al (College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Objective] The multiplication of Philadelphus coronarius 'Aureus' was sped up for its extensive application through the experiment.Method] The best media for the stem-tip and tender stem segment of axillary bud of Philadephus coronarius 'Aureus' as explants culturing was experimented.Result] The results showed that initial medium of Philadephus coronarius 'Aureus' was MS+NAA 0.05 mg/L+6-BA 0.50 mg/L with the induction rate of 100%;the best proliferation medium was MS+NAA 0.05 mg/L+6-BA 0.50 mg/L with the... |
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| Keywords: | Philadephus coronarius 'Aureus' Stem-tip Stem segment Tissue culture |
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