鸵鸟皮肤抗菌肽对雏鸡免疫器官指数及T淋巴细胞数量的影响初探

摘要：为了观察非洲鸵鸟皮肤抗菌肽对雏鸡免疫器官指数及T淋巴细胞数量的影响，进而研究其对雏鸡免疫系统的调节能力。选取50只1日龄健康雏鸡，随机分为两组：试验组（T）从1日龄开始每天在饮水中加入浓度为1μg/ml的鸵鸟皮肤抗菌肽提取物，对照组（C）饮水中不添加抗菌肽提取物，分别于1、4、7、10、17日龄时随机抽取5只雏鸡称重后处死，采取免疫器官称重并运用ANAE染色方法测定免疫器官T淋巴细胞数量。结果发现抗菌肽能够提高10~17日龄雏鸡体重，提高4~7日龄雏鸡的免疫器官指数及7日龄免疫器官的T淋巴细胞数量( P < 0.05)。结果表明饮水中添加非洲鸵鸟皮肤抗菌肽可以促进促进雏鸡免疫器官发育，增强机体的细胞免疫。

The preliminary study of antimicrobial peptides extracted from African Ostrich skin on the immune organs indexes and the number of T lymphocytes in immune organs of chickens

Abstract: To study the immunomodulation function of antimicrobial peptides extracted from African Ostrich skin (Ost-AvBD- skin) on chicken, the immune organs indexes and the number of T lymphocytes in immune organs were observed. Fifty chickens were randomly divided into treatment and control groups. In the treatment group chickens received drinking water supplemented with African Ostrich skin antimicrobial peptides (1μg/ml) right after hatching. Immune organs were taken at day 1, 4, 7, 10 and 17 respectively. African Ostrich skin antimicrobial peptides supplementation enhanced the weight of chickens from day 10 to 17, immune organs indexes from day 4 to 7 and the number of T lymphocytes in immune organs at day 7 ( P<0.05) . The results showed that the antimicrobial peptides extracted from African Ostrich skin could promote the development of chicken immune organs and cell immunity.