Molecular cloning of canine nm23 cDNAs and their expression in normal and tumor tissues |
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Authors: | Takahashi Masashi Une Ryoma Fukushima Kenjiro Fujiki Makoto Misumi Kazuhiro Miyoshi Noriaki Endo Yasuyuki Ohishi Akihiro Akuzawa Masao |
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Institution: | Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Kagoshima University, Japan. |
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Abstract: | Two canine nm-23 cDNAs, designated as nm23-C1 and -C2, were isolated and characterized. Both have a putative open reading frame consisting of 459-bp encoding 152 amino acids and are highly similar to human, mouse and rat homologues. To understand the potential role of nm23-C1 and -C2 in the development of mammary gland tumors (MGT), we analyzed the mRNA expression in 14 MGT samples by RT-PCR. The samples were divided into categories according to their histopathology (benign/malignant) and metastasis. No significant difference in the mRNA expression levels of either nm23-C1 or -C2 were observed between the benign and malignant groups or the metastatic and non-metastatic groups. These results suggest that nm23-C1 and -C2 are not related to the establishment of malignancy and metastatic lesions in canine MGT cases. |
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