Identification of AFLP Markers that Discriminate Between Cultivated
Cotton and the Hawaiian Island Endemic, <Emphasis Type="Italic">Gossypium tomentosum</Emphasis>Nuttall ex Seeman |
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Authors: | J?S?Hawkins J?Pleasants Email author" target="_blank">J?F?WendelEmail author |
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Institution: | (1) Department of Ecology, Evolution, and Organismal Biology, Iowa State University, 353 Bessey Hall, 50011 Ames, IA, USA |
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Abstract: | Interspecific gene flow from crop species into wild relatives may compromise the genetic integrity of native species and in
the case of transgenic crops lead to the escape of transgenes into natural populations. Approximately 72% of the upland cotton
(Gossypium hirsutum L.) acreage grown in the United States in 2000 utilized transgenic cultivars. The closest relative of G. hirsutum is G. tomentosum Nuttall ex Seeman, endemic to the Hawaiian archipelago. Because these two species are fully interfertile, cultivation of
transgenic G. hirsutum in the Hawaiian Islands is restricted. We investigated the possibility of identifying AFLP genetic markers that are diagnostic
for each species and thus could be used in future studies to detect introgression between them. In addition, we were interested
in comparing levels and geographic patterns of AFLP diversity in G. tomentosum to previous estimates using allozyme data. AFLP analysis led to the detection of 11 and 16 species-specific markers for G. tomentosum and G. hirsutum, respectively. These species-specific AFLP markers will be useful for detecting gene flow between G. hirsutum and G. tomentosum that has occurred in the past and thus might occur in the future if the restrictions on cultivation of transgenic G. hirsutum are relaxed in the Hawaiian Islands. Little genetic diversity and limited geographic patterning were discovered using AFLP
markers, consistent with data from previous allozyme studies. |
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Keywords: | AFLP Cotton Gene flow Genetic diversity Genetic marker Introgression Gossypium |
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