海洋芽胞杆菌B-9987菌株对番茄灰霉病和早疫病的作用机制初探

本文以分离自渤海潮间带海洋芽胞杆菌（Bacillus marinus）B〖CD*2〗9987为研究对象，在番茄活体和离体条件下，初步研究了B 9987菌株对番茄灰霉病和早疫病的防治作用及其机制。试验结果表明，B 9987菌株活菌液处理，表现最强的抑菌和控制病害的作用，其中对番茄灰葡萄孢菌和茄链格孢菌最低抑菌浓度均为4 μL/mL，对两种病害的生防效果分别达85.1%和88.4%；各处理番茄植株5种防御酶活性均显著增加，其中活菌原液处理诱导效果最好，对苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)及超氧化物歧化酶(SOD)活性表现为最高，分别为对照的4.13、2.54、1.85倍。表明B 9987菌株对番茄病害的防治是抑菌和诱导抗性综合作用的结果。

A primary study on the biocontrol mechanisms of Bacillus marinus B-9987 against the tomato gray mold and early blight

The biocontrol mechanisms and the fungistatic activities of the Bacillus marinus B-9987 were preliminarily studied under various treatments.The results showed that the fermentation broth of B-9987 had the highest activity in both fungitoxicity and controlling diseases.The minimum inhibitory concentration(MIC)to both Alternaria solani and Botrytis cirterea was 4 μL/mL and the control effect was 85.1%and 88.4%,respectively.The activities of five defense enzymes in each treatment were significantly improved.The fermentation broth had the highest induction activities to phenylalanine ammonialyase(PAL),polyphenol oxidase(PPO)and superoxide dismutase(SOD),with 4.13,2.54 and 1.85 times higher activities than the control,respectively.These data indicated that the biocontrol effect of B-9987 111ight be resulted from the synergetic effect of its antifungal activity and the induced resistance of the plant.