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菊花新品系叶片外植体不定芽再生体系的研究
引用本文:冯君伟,佟友丽,赵飞,李玉花. 菊花新品系叶片外植体不定芽再生体系的研究[J]. 北方园艺, 2009, 0(2)
作者姓名:冯君伟  佟友丽  赵飞  李玉花
作者单位:东北林业大学,黑龙江,哈尔滨,150040;山东农业大学,山东,青岛,266000
基金项目:国家高技术研究发展计划(863计划),东北林业大学校立科研基金 
摘    要:以'东林4号'、'东林6号'、'东林9号'3个优良菊花品系叶片外植体为试材,探讨了基因型、叶龄、潮霉素浓度等因素对菊花叶片不定芽再生的影响.结果表明:基因型是影响不定芽再生的重要因素,不同品系叶片不定芽再生的激素配比存在明显差异;同一品系,上层幼嫩叶片分化率明显高于中下部.'东林9号'品系叶片不定芽分化率高达90%,'东林9号'叶片及不定芽在30 mg/L的潮霉素中表现为全部死亡.东林4号最适分化培养基为:MS+3 mg/L BA+0.1 mg/L 2,4-D;东林6号最适分化培养基为:MS+2 mg/L BA+1mg/L NAA;东林9号最适分化培养基为:MS+0.5mg/L BA+1.5 mg/L NAA.

关 键 词:菊花  叶片  再生  外植体

Establishment of Shoot Regeneration System from Leaves of New Variety of Dendranthema grandiflora
FENG Jun-wei,TONG You-li,ZHAO Fei,LI Yu-hua. Establishment of Shoot Regeneration System from Leaves of New Variety of Dendranthema grandiflora[J]. Northern Horticulture, 2009, 0(2)
Authors:FENG Jun-wei  TONG You-li  ZHAO Fei  LI Yu-hua
Affiliation:1.Northeast Forestry University;Harbin;Heilongjiang 150040;China;2.Shandong Agricultral University;Qingdao;Shandong 266000;China
Abstract:Chrysanthemum of 'Donglin 4','Donglin 6' and 'Donglin 9'were used for tissue culture.The effect such as genotype,seedling age,density of hygromycin on shoot regenerate leaves from the lines of chrysanthemum was studied.The results were as followed: the genotype was the important factor in shoot regeneration,shoot regeneration in different hormone combination and different lines existed obvious discrimination.The bud differentiation rate of upper young leaf was higher than middle and lower parts of stem.The ...
Keywords:Chrysanthemum  Leaf  Regeneration  Explant  
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