白花草木樨半同胞家系的生物固氮性状评价

以不同的白花草木樨(Melilotus albus)半同胞家系为试验材料,分别种植于甘肃临泽和榆中,测定其根干重、根瘤数、根瘤重、叶全氮、叶全磷和生物固氮百分率,以此评价其生物固氮能力。结果表明,白花草木樨在两个地区表现出的各项固氮指标变异系数均大于15%,主成分分析后,可将半同胞家系划分为4组,组1白花草木樨家系的根干重、根瘤数、根瘤重、叶全氮、叶全磷和生物固氮百分率分别高出对照当地白花草木樨品种(RX-02) 4%、26%、10%、17%、24%、11%,其中生物固氮百分率的最大值和最小值分别为58%和43%。本研究筛选出的优良生物固氮性状的后代家系,可为进一步选育生物固氮能力强的新品种奠定基础。

Evaluation of traits related to the biological nitrogen fixation in Melilotus albus half-sib families

In this study,half-sib families of Melilotus albus populations were established in two different locations(Yuzhong and Linze)and their phenotypic variations in relation to biological nitrogen fixation(BNF)were evaluated.Root dry weight(RW),number of nodules per plant(NN),nodule weight(NW),leaf total nitrogen(LTN),leaf total phosphorus(LTP)and the percentage of nitrogen derived from the atmosphere(%Ndfa)in plant leaves were measured.The results showed that the coefficients of variation for these indices were greater than 15%,and principal component analysis identified four groups of half-sib families.The RW,NN,NW,LTN,LTP,and%Ndfa values of group 1 were 4%,26%,10%,17%,21%,and 11%higher,respectively,than in the control(RX-02).The maximum and minimum values of%Ndfa for half-sib families were 58%and 43%,respectively.The progeny families with good biological nitrogen fixation traits selected in this study can lay a foundation for further breeding of new varieties with strong biological nitrogen fixation ability。