微生物菌剂对伊犁绢蒿种子发芽特征的影响

为提高野外补播伊犁绢蒿(Seriphidium transiliense)幼苗的存活率,研究不同稀释倍数下(10、100、1000、3000、5000、10000倍)微生物菌剂对其发芽率、发芽势、根长、芽长及单株幼苗重的影响,并结合隶属函数法筛选最适宜的种子处理方式。结果表明,除复合芽孢杆菌抑制发芽率、发芽势外,适宜稀释倍数的生物菌剂均可提高伊犁绢蒿种子的发芽率、发芽势、根长、芽长及单株幼苗重。隶属函数综合评价认为,菌剂对该种子发芽及幼苗生长的促进效果由大到小依次为地衣芽孢杆菌、多粘类芽孢杆菌、复合芽孢杆菌、解淀粉芽孢杆菌、农用复合微生物菌、EM菌和放线菌。结合盆栽结果从微生物菌剂组合看,地衣芽孢杆菌稀释1000倍、10倍、5000倍及多粘类芽孢杆菌稀释1000倍对伊犁绢蒿种子整体萌发生长提升效果较好,可作为补播前种子处理的首选措施。

Effects of microbial inoculant on Seriphidium transiliense seed germination characteristics

To ensure reseeding survival rate,we measured the effects of different dilution multiples(×10,×100,×1000,×3000,×5000,×10000)of a variety of microbial inoculants on seed germination percentage,germination energy,root length,bud length,and seedling weight per plant of Seriphidium transiliense.Suitable seed treatment methods were then selected using principal component analysis and membership functions.The results showed that seed germination percentage,germination energy,root length,bud length,and seedling weight per S.transiliense plant increased with suitable dilution multiples of microbial inoculants;further,compared with the control,these indexes were significantly higher or did not differ significantly.The only exception was compound Bacillus,which decreased the seed germination and germination energy.According to a comprehensive evaluation of the membership functions,the microbial inoculants promoted seed germination and seedling growth in the following descending order:B.licheniformis,Paenibacillus polymyxa bacillus,compound B.,Amyloliquefaciens,agricultural compound microorganism bacteria,Actinomyces,and EM bacteria.Combined with the potted results of microbial inoculant,B.licheniformis×1000,B.licheniformis×10,B.licheniformis×5000,and Paenibacillus polymyxa×1000 were better at improving the germination and growth of S.transiliense as a whole and should the first choices for seed treatment before reseeding.