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| 黄曲霉毒素B1单克隆抗体的制备与鉴定 | |
| 引用本文： | 于师宇,王蓓蕾,谢光洪,李小兵,宋文学,周晓翠,张志刚,孔涛,张焱,肖成蕊,牟俊,于光,王哲.黄曲霉毒素B1单克隆抗体的制备与鉴定[J].安徽农业科学,2008,36(13):5465-5467. |
| 作者姓名： | 于师宇 王蓓蕾 谢光洪 李小兵 宋文学 周晓翠 张志刚 孔涛 张焱 肖成蕊 牟俊 于光 王哲 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春,130062;福建出入境检验检疫局,福建福州,350001 2. 长春生物制品研究所,吉林长春,130062 3. 吉林大学畜牧兽医学院,吉林长春,130062 4. 吉林出入境检验检疫局,吉林长春,130062 |
| 基金项目： | 国家质量检验总局科技计划项目 |
| 摘 要： | 目的]为黄曲霉毒素类生物毒素研究提供试验参考。方法]黄曲霉毒素B1与人血清白蛋白耦联后免疫BALB/c小鼠,取小鼠脾细胞与小鼠骨髓瘤细胞进行细胞融合,通过阳性株的筛选和克隆等步骤,制备了3株稳定分泌抗黄曲霉毒素B1的单克隆抗体,用酶联免疫吸附试验测定抗体的效价和特异性。结果]成功地筛选出能够分泌抗黄曲霉毒素B1的单克隆抗体杂交瘤细胞株,制备出对黄曲霉毒素B1具有高亲合力、高特异性的单克隆抗体。结论]为鲜奶及奶粉的快速筛检提供了技术支持。 |
| 关 键 词： | 黄曲霉毒素B1 单克隆抗体 杂交瘤细胞株 |
| 文章编号： | 0517-6611(2008)13-05465-02 |
| 修稿时间： | 2008年4月16日 |
Preparation and Identiffication of Monoclonal Antibody for Aflatoxin B1 |
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| YU Shi-yu.Preparation and Identiffication of Monoclonal Antibody for Aflatoxin B1[J].Journal of Anhui Agricultural Sciences,2008,36(13):5465-5467. | |
| Authors: | YU Shi-yu |
| Abstract: | Objective] The research aimed to provide experimental references for the study of biotoxins like aflatoxin.Method] After coupled with human serum albumin,aflatoxin B1 was used to immune BALB/c mice.The spleencells of mice was taken out and fused with mouse myeloma cells.Three monoclonal antibodies secreted anti aflatoxin B1 were preparated by steps of selection and clone and the efficiency and specifity of antibodies was tested by enzyme-linked immunosorbent assay.Results] Hybridoma cell lines secreted anti aflatoxin B1 were screened out successfully and monoclonal antibodies had high affinity and specificity with aflatoxin B1 was preparated.Conclusion] The study provided technical support for the quick screening of milk and milk powder. |
| Keywords: | Aflatoxin Monoclonal antibody Hybridoma cell lines |
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