Expression of small stress protein hsp20 gene in the maturing rat testis |
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Authors: | Yamano Yoshiaki Ohyama Kenji Ohta Masanori Nakamura Juri Morishima Isao |
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Institution: | Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University, Japan. |
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Abstract: | In this study, we cloned a cDNA that encodes a small heat shock protein, Hsp20 (alphaB crystallin-related protein), from a maturing rat testis by means of differential display. The full-length cDNA sequence was completely identical to that registered in the DNA databank. The expression of Hsp20 gene was detected strongly in the heart and slightly in the testis of a 9-week-old rat. The expression of Hsp20 increased gradually from three weeks to 9 weeks, and the strongest expression was observed in the testis at week fifteen. The expression was localized in spermatocytes and round spermatids. The gene expression was not affected by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) when it was administered into male rats during the nursling period. |
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