| 槭叶草离体快繁的研究 |
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| 引用本文： | 王克凤,赵春莉,陈立飞,赵和祥,董然,顾德峰.槭叶草离体快繁的研究[J].北方园艺,2012(5):135-136. |
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| 作者姓名： | 王克凤 赵春莉 陈立飞 赵和祥 董然 顾德峰 |
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| 作者单位： | 吉林农业大学园艺学院,吉林 长春,130118;吉林农业大学园艺学院,吉林 长春,130118;吉林农业大学园艺学院,吉林 长春,130118;吉林农业大学园艺学院,吉林 长春,130118;吉林农业大学园艺学院,吉林 长春,130118;吉林农业大学园艺学院,吉林 长春,130118 |
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| 基金项目： | 吉林省科学技术厅资助项目(20100259) |
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| 摘 要： | 以槭叶草实生无菌苗为外植体,MS培养基为基础,通过添加不同种类和浓度的激素,筛选出槭叶草组织培养的最适培养基。结果表明:种子较好的灭菌时间为0.1%升汞6min;增殖的最适培养基为MS+6-BA 0.1g/L+NAA 0.1g/L,其增殖倍数高达5.1,并且苗生长状态良好;生根最适培养基为1/2MS。
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| 关 键 词： | 槭叶草 实生苗 组织培养 |
Study on Rapid Propagation in vitro of Mukdenia rossii (Oliv.) Koidz |
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| WANG Ke-feng
,
ZHAO Chun-li
,
CHEN Li-fei
,
ZHAO He-xiang
,
DONG Ran
,
GU De-feng.Study on Rapid Propagation in vitro of Mukdenia rossii (Oliv.) Koidz[J].Northern Horticulture,2012(5):135-136. |
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| Authors: | WANG Ke-feng ZHAO Chun-li CHEN Li-fei ZHAO He-xiang DONG Ran GU De-feng |
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| Institution: | (College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | The experiment was based on MS culture media and Mukdenia rossii(Oliv.)Koidz.Used explants were from seedlings of the germination seeds in vitro.An appropriate culture medium was obtained via regulating the concentrations of cytokinin and auxin.The optimal sterilization method for seeds Mukdenia rossii(Oliv.)Koidz was 1% HgCl2 6 min.The propagation coefficient of 5.1 times indicated that MS+6-BA 0.1 mg/ L+NAA 0.1 mg/ L was the optimal culture medium for subculture proliferation.The best rooting medium was 1/2MS.The survival rate of the plantlets was shown to be up to 97%. |
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| Keywords: | Mukdenia rossii(Oliv )Koidz seedling tissue culture |
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