山楂叶片过氧化物同工酶分析

应用聚丙烯酰胺凝胶电泳(PAGE)技术,对山东省内24个山楂栽培品种样本进行过氧化物酶同工酶电泳分析,对获得的酶谱带和迁移率进行分析,并通过聚类分析法对其亲缘关系进行研究。结果表明:24个样本之间有1条共同的同工酶谱带存在17条特异性的条带,说明供试样本之间虽然有一定的共同起源,但是在进化过程中逐渐产生差异。根据分析结果将供试的24个栽培品种分为3个聚类群组以表示各品种的亲缘关系的远近;各品种之间的亲缘关系对品种选育有重要的指导意义。

Peroxide Isozyme Analysis of Hawthorn Leaf

With the techniques of polycrylamid gel electrophoresis the peroxide isozyme of 24 cultivar samples were detected and analyzed.These 24 cultivar samples were collected from four different district in Province of Shandong.The enzyme bands and migration rate obtained by polycrylamid gel electrophoresis were studied.And the genetic relationship was analyzed through the clustering analysis.The results showed that there are 1 common band and 17 specific bands among 24 cutivar samples.It indicated that these 24 cultivar samples had common origin to a certain extent,but in the evolutionary process differences gradually generated among these samples.According to the results of analysis 24 cultivated varieties were divided into three clustering group.They demonstrated the genetic relationship among the varieties.The genetic relationship among the varieties had guiding significance for breeding.