一品红组培苗快繁技术的研究

以一品红带芽茎段、叶片为外植体,研究了不同激素浓度配比的MS培养基对愈伤组织、芽的分化及增殖和试管苗生根的不同效应。结果表明:最佳愈伤组织诱导培养基为MS+BA0.2mg/L+NAA 0.5mg/L+蔗糖30g/L+琼脂7.5g/L(pH 5.6),诱导率达到92.5%;芽的分化最佳培养基为MS+BA 2.0mg/L+NAA 0.2mg/L+蔗糖30g/L+琼脂7.5g/L,不定芽的诱导率达到83.3%;芽的增殖培养基为MS+BA 1.0mg/L+NAA 0.2mg/L+蔗糖30g/L+琼脂5g/L,增殖系数为10.8;而最佳生根培养基为1/2MS+BA 0.2mg/L+NAA 0.5mg/L+蔗糖30g/L+琼脂7.5g/L,每芽生根数平均为3.5条。该研究结果为一品红组培苗的工厂化生产提供了可靠的技术指标。

Study on the Tissue Culture Technology of the Seedling Propagation in Euphorbia pulcherrima Wild

Using leaf and stem with axilla bud of Euphorbia pulcherrima Wild as the explant respectively,effects on the callus induction,the shoot differentiation and propagation,and the root differentiation of different phytohormone combition in MS basic medium were studied.The results showed that the best medium for callus induction was MS+BA 0.2 mg/L+NAA 0.5 mg/L,and the differentiation rate reached 92.5 %;The medium MS+BA 2.0 mg/L+NAA 0.2 mg/L was the most suitable for shoot induction than others,and the differentiation rate reached 83.3%;The medium with MS+BA 1.0 mg/L+NAA 0.2 mg/L was the best one for shoot propagation,the propagation rate was 10.8;Whereas,the best medium for root differentiation was 1/2 MS+BA 0.2 mg/L+NAA 0.5 mg/L,and the average root number per shoot was 3.5.This result would was beneficial to culture the Euphorbia pulcherrima Wild seedlings industrially.