| 怀地黄愈伤组织诱导及细胞悬浮培养体系的建立 |
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| 引用本文： | 张晓丽,王建军,徐守真,李敬敬,陈明霞,李明军.怀地黄愈伤组织诱导及细胞悬浮培养体系的建立[J].北方园艺,2012(1):162-164. |
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| 作者姓名： | 张晓丽 王建军 徐守真 李敬敬 陈明霞 李明军 |
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| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡453007;河南省高校道地中药材保育及利用工程技术研究中心,河南新乡453007
2. 河南师范大学生命科学学院,河南新乡,453007 |
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| 基金项目： | 国家农业科技成果转化资金资助项目(2009D00010539);河南省教育厅科技攻关资助项目(2010A180009) |
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| 摘 要： | 研究了植物生长调节剂和接种方式对怀地黄叶片愈伤组织诱导的影响和细胞悬浮培养体系的建立。结果表明:将叶片正接在MS+2,4-D 1mg/L+6-BA 0.4mg/L培养基上,愈伤组织生长良好,诱导率达100%;在该培养基上多次继代,可形成3种类型的愈伤组织,其中Ⅰ型愈伤组织淡黄色、颗粒透亮、分散性好且疏松易脆,适合进行细胞悬浮培养;在MS+2,4-D 3mg/L+6-BA 0.5mg/L培养基中,悬浮培养细胞生长曲线呈"S"型,培养15d时细胞干重达最大值,为4.94g/L。
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| 关 键 词： | 怀地黄 愈伤组织 细胞悬浮培养 |
Research on Callus Induction and Cell Suspension Culture of Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao |
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| ZHANG Xiao-li
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WANG Jian-jun
,
XU Shou-zhen
,
LI Jing-jing
,
CHEN Ming-xia
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LI Ming-jun.Research on Callus Induction and Cell Suspension Culture of Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao[J].Northern Horticulture,2012(1):162-164. |
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| Authors: | ZHANG Xiao-li WANG Jian-jun XU Shou-zhen LI Jing-jing CHEN Ming-xia LI Ming-jun |
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| Institution: | 1,2(1.College of Life Science,Henan Normal University,Xinxiang,Henan 453007;2.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,University of Henan Province,Xinxiang,Henan 453007) |
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| Abstract: | The effects of inoculation method and plant growth regulator on callus induction and establishment of cell suspension culture system were studied.The rusults showed that when upper surface of leaf contact the medium,callus grows well on MS+2,4-D 1 mg/L+6-BA 0.4 mg/L,induction rate was 100%;on the same medium,callus could be divided into 3 types,callus of typeⅠwas straw yellow,bright particles,well dispersed and granular,fragile,this type of callus was suitable for cell suspension culture;on MS+2,4-D 3 mg/L+6-BA 0.5 mg/L,the growth curve of suspension culture cells were ’S’ type,cells dry weight was 4.94 g/L when cells cultured for 15 days. |
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| Keywords: | Rehmannia glutinosa f hueichingensis(Chan et Sehih) Hsiao callus cell suspension culture |
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