| 病毒诱导的基因沉默在植物中的应用现状 |
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| 引用本文： | 刘海萍.病毒诱导的基因沉默在植物中的应用现状[J].北方园艺,2012(4):171-173. |
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| 作者姓名： | 刘海萍 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 国家自然科学基金资助项目(31000925) |
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| 摘 要： | 传统的病毒诱导的基因沉默(Virus induced gene silencing,VIGS)是一种小干扰RNA(Small interfering RNAs,siRNA)介导的RNA沉默技术(SIR VIGS),作为一种有效的反向遗传学技术广泛的用于植物基因组功能的鉴定。另外,VIGS技术开始用来研究植物内源miRNA(microRNA)的功能,这为miRNA的功能研究提供了快速有效的方法。随着基因沉默技术的应用和改进,将会有越来越多的植物基因组功能得到快速有效的验证。
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| 关 键 词： | 病毒诱导的基因沉默 沉默载体 小干扰RNA miRNA |
The Application Status of Virus Induced Gene Silencing (VIGS) in Plant |
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| LIU Hai-ping.The Application Status of Virus Induced Gene Silencing (VIGS) in Plant[J].Northern Horticulture,2012(4):171-173. |
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| Authors: | LIU Hai-ping |
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| Institution: | LIU Hai-ping (College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083) |
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| Abstract: | Small interfere RNA plays the main role in traditional virus induced gene silencing which is a reverse genetics technology and has been widely used in plants to study gene function.Besides,VIGS also provides a rapid and effective method in researching function of miRNA.As the development of VIGS,more and more gene function will be validated in plant in the future. |
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| Keywords: | virus induced gene silencing vectors small interfere RNA microRNA |
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