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天人菊菌核病病原鉴定及其拮抗木霉菌株的筛选
引用本文:刘威,叶云峰,李林轩,蒋妮,刘丽辉.天人菊菌核病病原鉴定及其拮抗木霉菌株的筛选[J].北方园艺,2012(5):142-145.
作者姓名:刘威  叶云峰  李林轩  蒋妮  刘丽辉
作者单位:广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室,广西南宁 530023;广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室,广西南宁 530023;广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室,广西南宁 530023;广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室,广西南宁 530023;广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室,广西南宁 530023
基金项目:广西药用植物园青年基金资助项目(桂药基200807)
摘    要:通过致病性测定、病害症状、病原菌培养性状及形态特征等对天人菊菌核病病原菌进行鉴定;通过室内拮抗作用测定和盆栽防治效果测定筛选生防木霉菌株。结果表明:将天人菊菌核病的病原菌鉴定为核盘菌(Sclerotinia sclerotiorum(Lib)de Bary);筛选到对天人菊菌核病菌拮抗作用最强,盆栽防治效果最好的菌株是哈茨木霉H5,其拮抗指数为Ⅰ级,防治效果为93.2%。在测试的5个木霉菌株中,哈茨木霉H5对天人菊菌核病的防治效果最好。

关 键 词:天人菊  菌核病  病原鉴定  拮抗木霉  筛选

Identification of the Pathogen of Sclerotiniose on Gaillardia pulchella and Screening of Antagonistic Trichoderma Strain
LIU Wei , YE Yun-feng , LI Lin-xuan , JIANG Ni , LIU Li-hui.Identification of the Pathogen of Sclerotiniose on Gaillardia pulchella and Screening of Antagonistic Trichoderma Strain[J].Northern Horticulture,2012(5):142-145.
Authors:LIU Wei  YE Yun-feng  LI Lin-xuan  JIANG Ni  LIU Li-hui
Institution:(Guangxi Botanical Garden of Medicinal Plant,Guangxi Medicinal Resources Conservation and Genetic Improvement Laboratory,Nanning,Guangxi 530023)
Abstract:The pathogen was identified based on pathogenicity,disease symptom,cultivation characters and morphology.The antagonistic Trichoderma strain was screened by antagonistic ability tests in vitro and pot experiments.The results showed that pathogen of sclerotiniose on Gaillardia pulchella was identified as Sclerotinia sclerotiorum(Lib) de Bary.Trichoderma harzianum H5 gave the strongest antagonistic ability in vitro and highest control efficiency in pot experiments.Its antagonistic index and control efficiency were Ⅰ and 93.2%,respectively.Among the five tested Trichoderma strains,Trichoderma harzianum H5 had the best control potential for controlling the sclerotiniose on Gaillardia pulchella.
Keywords:Gaillardia pulchella  sclerotiniose  identification of the pathogen  antagonistic Trichoderma strain  screen
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