珍稀濒危莲瓣兰组织培养试管苗移栽的研究

以莲瓣兰‘苍江大雪素’组织培养生根试管苗为试材,研究移栽基质、移栽时间、试管苗大小对莲瓣兰试管苗移栽的影响。结果表明:在大理3月和9月份莲瓣兰试管苗移栽成活率最高,二者无显著差异,3月份移栽根系生长显著优于9月份,是一年中移栽的最佳时期;栗树叶作为移栽基质莲瓣兰试管苗移栽成活率和生根显著优于水苔、珍珠岩、红仙土和碎树皮,水苔、珍珠岩和红仙土不宜作为莲瓣兰试管苗的移栽基质;莲瓣兰试管苗大小对移栽成活率及生根影响较大,中苗移栽成活率显著优于大苗和小苗,大苗移栽生根优于中苗,小苗移栽难生根。

Study on Transplant of Tissue Culture Test-tube Plantlet of Rare and Endangered Plant Cymbidium tortisepalum

Taking the test-tube plantlet of Cymbidium tortisepalum’Cangjiangdaxuesu’ after tissue culture seedling as test materials,the effect of transplant substrate,transplant time and the size of test-tube plantlet were studied.The results showed that the transplant survival rate of Cymbidium tortisepalum test-tube plantlet was the highest in March and September in Dali.They were no significant deviation.March was the best transplant period,in this period the radication exceeded September markedly;using shredded bark as transplant base material,the survival rate and radication of test-tube plantlet were better than others,including water musci,perlite,hongxian soil and chestnut leave,so the water musci,perlite and hongxian soil were not situable as transplant base material;the size of test-tube plantlet had further effect on the survival rate and radication,the transplant survival rate of middle seedlings was higher than big seedlings and small seedlings markedly,big seedlings’ were better than small seedlings,the small seedlings were hardly radicat.