三种玉簪新品种离体快繁技术的研究

以3种玉簪"金头饰"(H.‘Gloden Tiaia’)、"小黄金叶"(H.‘Gloden Cadet’)和"金鹰"(H.‘Gloden Eagle’)的幼嫩花蕾为外植体诱导不定芽,成功的建立了3个品种的离体快繁体系。结果表明:不定芽诱导最佳培养基为MS+6-BA 2.5mg/L+NAA 0.2mg/L;在增殖培养基中,"金头饰"最佳增殖培养基为MS+6-BA 2.0mg/L+NAA 0.5mg/L;"小黄金叶"最佳增殖培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L;"金鹰"最佳增殖培养基MS+6-BA 4.0mg/L+NAA0.2mg/L;无生长调节剂的MS为"金鹰"的最佳生根培养基,生根率达100%以上;"小黄金叶"在培养基MS+NAA 0.5mg/L中生根率达到100%以上;"金头饰"在培养基MS+NAA 0.3mg/L中生根率也达到100%以上。其移栽成活率均为100%。

Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species

The young buds of H.’Gloden Tiaia’,H.’Gloden Gadet’and H.’Gloden Eagle’were used as the explants to develop a protocol for adventitious buds induced.The successful establishment of five species of rapid propagation system.The results showed that the best medium for adventitious buds induced was MS+6-BA 2.5 mg/L+ NAA 0.2 mg/L;In the proliferation culture,the best proliferation medium for H.’Gloden Tiaia’was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L;The best proliferation medium for H.’Gloden Gadet’was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;The best proliferation medium for H.’Gloden Eagle’was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L.Medium MS as H.’Gloden Eagle’rooting medium,the rooting rate was over 100%;H.’Gloden Gadet’in the rooting medium MS + NAA 0.5 mg/L;rooting rate was over 100%;H.’Gloden Tiaia’in the rooting medium MS + NAA 0.3 mg/L;rooting rate was over 100%.The transplant survival rate of 100%.