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有机氮对平菇原基分化期胞外酶的影响
引用本文:陈仁玉,刘朝贵,方琼.有机氮对平菇原基分化期胞外酶的影响[J].北方园艺,2012(1):153-155.
作者姓名:陈仁玉  刘朝贵  方琼
作者单位:西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆400715
摘    要:以平菇为试材,采用不同有机氮浓度的培养料培养,在平菇原基形成的扭结期、桑椹期、珊瑚期分别测定漆酶、愈创木酚氧化酶、邻苯二酚氧化酶、淀粉酶、羧甲基纤维素酶的活性。结果表明:配方5(棉籽壳97.70%,尿素0.30%,过磷酸钙1%,糖1%)是最好的配方,胞外酶活性最大,平菇子实体分化速度快;适宜的有机氮含量有助于增加平菇原基分化过程中各酶活性,加快原基分化进程。

关 键 词:平菇  有机氮  原基分化  酶活性

Effects of Organic Nitrogen on the Activity of Extracellular Enzymes During Primordium Formation of Pleurotus ostreatus
CHEN Ren-yu , LIU Chao-gui , FANG Qiong.Effects of Organic Nitrogen on the Activity of Extracellular Enzymes During Primordium Formation of Pleurotus ostreatus[J].Northern Horticulture,2012(1):153-155.
Authors:CHEN Ren-yu  LIU Chao-gui  FANG Qiong
Institution:(College of Horticulture and Landscape Architecture,Southwest University,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Key Laboratory of Olericulture,Chongqing 400715)
Abstract:Pleurotus ostreatus was used as material in this study to test activity of laccase,guaiacol oxidase,pyrocatechol oxidase,amylase and carboxymethyl cellulase with medium of various organic nitrogen content in primordia stage,morula stage and coral period.The results showed that formula 5(Sawdust 97.70%,urea 3%,calcium superphosphate 1%,glucose 1%) was the best option with maximum exocellular enzymatic activity and fastest differentiation.A suitable content of organic nitrogen was good for promoting enzymatic activity during primordium formation of Pleurotus ostreatus,and speeded up the process of primordium formation.
Keywords:Pleurotus ostreatus  organic nitrogen  primordium formation  enzyme activity
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