| 怀地黄花药培养影响因素的初步研究 |
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| 引用本文： | 周春娥,朱凤荣,周延清.怀地黄花药培养影响因素的初步研究[J].北方园艺,2012(4):128-130. |
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| 作者姓名： | 周春娥 朱凤荣 周延清 |
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| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡,453007
2. 新乡学院生命科学与技术系,河南新乡,453000 |
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| 基金项目： | 国家高技术研究发展计划“863”项目子课题资助项目(2006AA100109);河南省教育厅自然科学研究计划资助项目(2009B180016);河南省教育厅自然科学研究计划资助项目(2011B180035);河南师范大学青年科学基金资助项目 |
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| 摘 要： | 以怀地黄花药为外植体,研究花药发育时期和激素对怀地黄花药愈伤组织诱导率和分化率的影响。结果表明:怀地黄花药在4℃条件下预处理3d后接种在含有2,4-D 2.0mg/L、6-BA 0.5mg/L和KT 2.0mg/L的MS培养基上对花药愈伤组织诱导最有利,而最适的愈伤组织分化培养基为MS+IAA 0.5mg/L+6-BA 1.0mg/L。
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| 关 键 词： | 怀地黄 花药培养 植株再生 |
Study on Influential Factors on Anther Culture of Rehmannia glutinosa Libosch |
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| ZHOU Chun-e
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ZHU Feng-rong
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ZHOU Yan-qing.Study on Influential Factors on Anther Culture of Rehmannia glutinosa Libosch[J].Northern Horticulture,2012(4):128-130. |
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| Authors: | ZHOU Chun-e ZHU Feng-rong ZHOU Yan-qing |
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| Institution: | 1 (1.College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007;2.Department of Life Science Biotechnology,Xinxiang University,Xingxiang,Henan 453000) |
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| Abstract: | Taking the anthers from Rehmannia glutinosa Libosch as explants,several factors such as the stage of anther and different hormone treatments were studied on anther callus induction and shoot differentiation induction.The results showed that the optimum callus induction could be obtained by inoculation on MS culture medium containing 2,4-D 2.0 mg/L,6-BA 0.5 mg/L and KT 2.0 mg/L after storage at 4℃ for 3 d,and the optimums shoot differentiation medium was MS+IAA 0.5 mg/L+6-BA 1.0 mg/L. |
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| Keywords: | Rehmannia glutinosa Libosch anther culture plant regeneration |
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