桑螟越冬幼虫体内蛋白质、氨基酸、碳水化合物的变化与抗寒性的关系

用分光光度法、液相色谱法及氨基酸测定仪等测定了桑螟越冬幼虫虫体、血淋巴中蛋白质、氨基酸、小分子碳水化合物等的含量变化,并分析了该类物质的含量变化与抗寒性的关系。结果表明:虫体与血淋巴中的蛋白质在越冬期间相互转移,过冷却能力变化与血淋巴蛋白质含量的增减一致,12月至翌年2月间是血淋巴蛋白质含量最高的时期,此期的过冷却点亦最低;丝氨酸、丙氨酸、酪氨酸、赖氨酸、精氨酸的含量变化趋势大致相同,含量与过冷却点的变化一致,随着寒冬的到来迅速上升,随着越冬期的结束又迅速下降,这5种氨基酸含量的变化,影响着桑螟幼虫的过冷却点和抗寒能力的变化;桑螟越冬幼虫以“小分子碳水化合物(海藻糖、甘露醇、山梨醇)氨基酸(丝氨酸,丙氨酸,酪氨酸,赖氨酸,精氨酸)甘油蛋白质结合脂肪”组成抗寒物质系统。

Relationship Between Variation of Protein,Amino Acid, Low-molecular Carbohydrate in Over-wintering Diaphania pyloalis Walker Larvae and Cold-hardiness

Content variation of protein,amino acid and low-molecular carbohydrate in the body and hemolymph of over-wintering larvae were measured by spectrophotometer, liquid chromatogram and amino acid measuring apparatus. Relation of these substance and cool-hardiness was analyzed. Results showed that protein transferred between body and hemolymph in the period of over-wintering. Super-cooling capability enhanced along with protein content of blood increasing, weakened along with decreasing. Content variety trends of Ser, Ala, Tyr, Lys and Arg were approximately same with and consistent the changing of supper cooling point. Content of these kinds of amino acid increased quickly in winter whereas decreased quickly along with ending of over-wintering. Contents of these five kinds of amino acids affected super-cooling point and cold-hardiness capability. The cold-hardiness substance system in Diaphania pyloalis Walker was made up of low-molecular carbohydrate(trehalose, mannitol and sorbitol)-amino acid (Ser, Ala, Tyr, Lys and Arg)-glycerin-protein-combined fat.