春兰菌根的显微结构观察

应用石蜡切片法,借助于光学显微镜观察春兰无菌组培苗、接菌组培苗的根及野生状态下的新生营养根、老根,结果表明:接菌组培苗的根及野生状态下的新生营养根、老根,其皮层组织细胞内有大量的菌丝结等兰科菌根的典型结构,而无菌组培苗的根则无菌丝、菌丝结等结构。菌根真菌自外皮层薄壁通道细胞侵入根的皮层细胞,春兰菌根是典型的地生兰菌根。

Observation of the Microstructure of Cymbidium goeringii Mycorrhiza

The microstructures of 4 types of Cymbidium goeringii roots, i.e., roots of seedlings produced from sterilized tissue culture, roots of seedlings produced from tissue culture inoculated with mycorrizal fungi, young nutritive roots and mature roots of the wild seedlings were observed separately by paraffinmethod with microscope.It was indicated by the observation that there were large quantity of pelotons in the cortex cells of both young nutritive roots andmature roots of either seedlings produced from inoculated tissue culture with mycorrizal fungi or wild seedlings of Cymbidium goeringii, showing typical structure of mycorrhiza, while there were no hyphae and pelotons observed in the cortex cells of roots out of seedlings produced from sterilized tissue culture.It was observed that the hyphae intruded into the roots of Cymbidium goeringii seedlings across the passage cells and entered the cortex cells.It was showed that the mycorrhiza of Cymbidium goeringii was typical orchidaceous mycoorhiza.