Development and characterization of wheat–Aegilopskotschyi1Uk(1A) substitution line with positive dough quality parameters |
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| Authors: | JIANG Yun WANG De-li HAO Ming ZHANG Jie LIU Deng-cai |
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| Institution: | 1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, P.R.China
3 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China |
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| Abstract: | Exploring novel high molecular weight glutenin subunits (HMW-GSs) from wild related species is a strategy to improve wheat processing quality. The objective of the present investigation was to identify the chromosomes of the wheat-alien introgression line N124, derived from the hybridization between Triticum aestivum with Aegilops kotschyi, and characterize the effects on quality-related traits. Fluorescence in situ hybridization karyotypes showed that N124 is a disomic 1Uk(1A) substitution line. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the Ae. kotschyi parent. PacBio RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were Ukx and Uky. Compared to the wheat parent, the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality parameters. It can be served as a donor or bridge material for wheat quality improvement. |
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| Keywords: | Aegilops kotschyi common wheat 1Uk(1A) substitution line HMW-GS processing quality |
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