玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达分析

【目的】分析研究玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达。【方法】使用NCBI Blast、DNAMAN和MotifScan等程序分析玉米钙依赖蛋白激酶CDPK的蛋白结构域和系统发育。采用Real-time RT-PCR方法分析CDPK基因在干旱胁迫处理玉米幼苗中的表达,评价CDPK基因对玉米干旱胁迫反应能力。【结果】鉴定出了在玉米中含有39个CDPK基因。将CDPK基因分为4个组。每个群体中的成员有共同的蛋白质基序和外显子及内含子结构,共同的进化起源。39个玉米CDPK基因根据它们的系统发育关系分组,并锚定在特定的玉米染色体上。多数CDPK基因在玉米干旱胁迫中的表达水平发生改变,CDPK基因参与对干旱胁迫的响应。【结论】从玉米基因组数据库中鉴定出了39个CDPK基因,大多数玉米CDPK基因在不同组织和不同发育阶段表现出不同的表达水平,CDPK基因在玉米发育中发挥不同的作用。多数CDPK基因在干旱胁迫下的表达量均发生变化。

Genome-wide identification of the maize calcium-dependent protein kinase and drought expression analysis of the CDPK gene family in maize

【Objective】Genome-wide identification of the maize calcium-dependent protein kinase and drought expression analysis of the CDPK gene family in maize.【Methods】NCBI Blast, DNAMAN and MotifScan were used to analyze the protein domains and phylogeny of CDPK in maize by genome-wide analysis. Real-time RT-PCR was performed to analyze the expression of CDPK gene in maize seedlings under drought stress, and to evaluate the effect of CDPK gene on maize response to drought stress.【Results】Through bioinformatics analysis of the whole genome of maize, 39 CDPK genes were identified in maize. According to phylogenetic analysis of CDPKs in maize, rice and Arabidopsis thaliana, these CDPK genes were divided into four groups. The members of each group share a common protein motif and exon and intron structure, suggesting that they may have a common evolutionary origin. The 39 maize CDPK genes are grouped according to their phylogenetic relationships and are anchored to specific maize chromosomes. It was found that the expression levels of most CDPK genes changed in maize under drought stress, indicating the response of these CDPK genes to drought stress.【Conclusion】39 CDPK genes were identified from maize genome database. Most of the CDPK genes in maize showed different expression levels in different tissues and different development stages, indicating that CDPK genes play different roles in maize development. At the same time, the expression levels of most CDPK genes changed under drought stress, which laid a foundation for further screening and studying the function of CDPK gene family under drought stress.