硼对两种不同砧木‘纽荷尔’脐橙叶片硼形态影响的差异

 【目的】阐明不同硼处理下枳橙砧木与枳壳砧木‘纽荷尔’脐橙细胞水平上的硼利用能力差异。【方法】采用水培试验,通过测定叶片中不同形态硼(自由态硼、半束缚态硼、束缚态硼)含量并分析其相互关系,研究两种砧木脐橙叶片硼形态的差异。【结果】在缺硼B0(B 0 mg?L-1)、正常硼B05 (B 0.5 mg?L-1)、高硼B50 (B 5.0 mg?L-1)条件下,枳橙砧木的自由态硼含量相对较低,半束缚态硼含量相对较高,枳壳砧木的正好相反;枳橙砧木在B0、B05、B50条件下的R值(半束缚态硼/自由态硼)分别为2.31、1.23和1.06,都远大于相同处理下枳壳砧木的1.80、0.57和0.82;高硼(B 5.0 mg?L-1)条件下,枳橙砧木的自由态硼和半束缚态硼分别为16.39、17.38 mg?kg-1, 都远大于枳壳砧木的8.60和7.02 mg?kg-1;对于束缚态硼,枳橙砧木的脐橙正常供硼与高硼条件下分别为7.34、7.43 mg?kg-1, 已没有显著差异,而枳壳砧木的则是高硼条件下最高,为9.20 mg?kg-1。【结论】与枳壳砧木相比,枳橙砧木脐橙体内硼的跨膜能力更强,细胞水平上的硼利用能力较高,在相对较低硼水平下就能满足细胞壁的需求。

Effect of Boron on Leaf Boron Forms of Newhall Navel Orange Grafted on Two Rootstocks

【Objective】To clarify the influence of boron (B) treatments on utilization ability of B in Newhall navel orange(Citrus sinensis Osbeck ‘Newhall’)grafted on citrange [C. sinensis (L.) Osb.× Poncirus trifoliata (L.) Raf.] or trifoliate orange [Poncirus trifoliata(L.)Raf.] at cellular level.【Method】Nutrient solution culture method was used in this study. The difference of leaf B forms between the two rootstocks was analyzed according to the concentration of B forms and their relative relationship.【Result】The results showed that the free B concentration of citrange was relatively lower and the sime-bound B concentration was relatively higher in the treatments of B deficiency (B0, B 0 mg?L-1), normal B (B05, B 0.5 mg?L-1) and high B (B50, B 5.0 mg?L-1), on the contrary, in trifoliate orange. R value of citrange was 2.31, 1.23, and 1.06 in the treatments of B0, B05, B50,respectively, which was much higher than that (1.80, 0.57, 0.82) of trifoliate orange in the same treatment. The free B and semi-bound B concentrations of citrange (16.39,17.38 mg?kg-1) were more higher than that of trifoliate orange (8.60 and 7.02 mg?kg-1) in the treatment of B50. Under the conditions of normal or high B, the bound B concentration of citrange was 7.34 and 7.43 mg?kg-1, respectively, there was no significant difference, while the bound B concentration of trifoliate orange was the highest (9.20 mg?kg-1) under the condition of high boron.【Conclusion】B transmembrane transport was easier in citrange-grafted plants compared to that in trifoliate orange, so B utilization ability of Newhall navel orange grafted on citrange was higher. Furthermore, B could fully bind the cell wall in citrange-grafted plants at relatively low B supply.