青花菜不同器官中4-甲基亚磺酰丁基硫苷及萝卜硫素含量分析

 【目的】青花菜的抗癌功能主要与4-甲基亚磺酰丁基硫苷的降解产物萝卜硫素有关,通过测定不同青花菜材料花球、茎和叶片中两者的含量并分析两者含量间的相关性,为青花菜高营养品质育种提供理论依据。【方法】采用反相高效液相色谱法,对24个不同青花菜材料花球、茎和叶片中4-甲基亚磺酰丁基硫苷含量,及其中的10份材料花球、茎和叶片中萝卜硫素含量进行测定和分析。【结果】青花菜不同基因型间以及不同器官间4-甲基亚磺酰丁基硫苷和萝卜硫素含量差异均极显著,4-甲基亚磺酰丁基硫苷和萝卜硫素含量均为花球含量最高,其次为茎,叶片含量最低;不同材料花球中4-甲基亚磺酰丁基硫苷平均含量分别为茎和叶片的4.4和13.97倍,萝卜硫素平均含量分别为茎和叶片的4和8.7倍,而有的基因型材料茎和叶片中4-甲基亚磺酰丁基硫苷的含量较高,约为花球中的50%;参试的10个不同基因型材料的花球、茎和叶片中的萝卜硫素含量与其前体4-甲基亚磺酰丁基硫苷含量间相关系数分别为0.9886**,0.9994**,0.9935**。【结论】不同青花菜材料间不同器官中4-甲基亚磺酰丁基硫苷含量存在显著差异;在青花菜萝卜硫素开发应用中,可选择其前体4-甲基亚磺酰丁基硫苷作为筛选指标,并可筛选茎和叶中4-甲基亚磺酰丁基硫苷含量较高的材料作为提取萝卜硫素的原料。

Analysis of Glucoraphanin and Sulforaphane Contents in Different Organs of Broccoli

【Objective】The anticarcinogenic activity of broccoli is most likely to be due to the activity of the isothiocyanates sulforaphane derived from glucoraphanin. In order to provide information in broccoli breeding for high nutrition quality, the goal of this study is to determine the contents of glucoraphanin and sulforaphane and analyze the correlation between them which is in the florets, stems and leaves of different genotypes of broccoli.【Result】Both glucoraphanin and sulforapane content difference was extremely significant in the florets, stems and leaves of different broccoli genotypes. The highest contents of them were both in the florets, while the lowest contents were in the leaves. The mean value of glucoraphanin content in the florets was 4.4- and 13.97-fold, respectively, higher than that in the stems and leaves, and the mean value of sulforaphane content in the florets was 4- and 8.7-fold, respectively, higher than that in the stems and leaves. However, compared to most materials, there were higher glucoraphanin contents in the stems and leaves of some broccoli materials about 50% of which in the florets. The correlation coefficient between the glucoraphanin and sulforaphane contents in the florets, stems and leaves of the ten different genotype broccoli materials were 0.9886**, 0.9994**, 0.9935**, respectively.【Conclusion】There exist some genotypic differences which can significant affect the glucoraphanin content in different genotype broccoli materials and organs. In the development and application of sulforaphane, the glucoraphanin contents can be the screening index. Broccoli materials which have higher glucoraphanin contents in the stems and leaves can be selected as the raw materials for extraction of the sulforaphane from the wastes.