霍山石斛组培丛生芽诱导增殖及生根技术

以霍山石斛无根试管苗为试材,研究了丛生芽诱导增殖和生根技术.丛生芽诱导增殖通过4因素3水平正交试验,筛选出的最适培养基为1/2MS 0.2 mg/LKT 0.2 mg/L NAA 0.3 mg/L 6-BA.4个因子对霍山石斛丛生芽诱导的影响顺序依次为:KT＞基本培养基＞6-BA＞NAA,其中,KT对霍山石斛丛生芽的诱导效果极为显著.添加不同浓度的生长素及香蕉泥进行生根诱导,其生根效果排序为:香蕉泥＞IBA＞NAA,最佳的生根培养基是MS基本培养基添加20%香蕉泥,生根率可达94.3%.

Studies on Clustered Shoots Induction, Proliferation and Rooting in Vitro of Dendrobium huoshanenese

The clustered shoots induction,proliferation and rooting techniques of tube Dendrobium huoshanenese seeding were developed in this paper.The optimal inducing and proliferating medium:1/2MS 0.2 mg/L KT 0.2 mg/L NAA 0.3 mg/L 6-BA,was selected by the orthogonal design of four factors and three levels.The results also showed that the orders of four factors to influence the tufted-bud induction of Dendrobium huoshanense were:KT> minimal medium >6-BA>NAA,and KT was the most effect on Dendrobium huoshanense.The orders of different concentration of auxin and banana mud added to induce root formation were:banana mud>IBA>NAA.The optimal rooting medium was MS with 20% banana mud,and rooting rate was 94.3%.