| 放线菌株D35的分离鉴定及其抗植物病原真菌活性 |
| |
| 引用本文： | 朱宏建,周倩,高必达. 放线菌株D35的分离鉴定及其抗植物病原真菌活性[J]. 湖南农业大学学报(自然科学版), 2009, 35(2): 138-141 |
| |
| 作者姓名： | 朱宏建 周倩 高必达 |
| |
| 作者单位： | 湖南农业大学,生物安全科学技术学院,湖南,长沙,410128;湖南农业大学,生物安全科学技术学院,湖南,长沙,410128;湖南农业大学,生物安全科学技术学院,湖南,长沙,410128 |
| |
| 基金项目： | 湖南省研究生科研创新项目，湖南农业大学人才稳定基金 |
| |
| 摘 要： | 从湖南壶瓶山国家自然保护区土样中分离放线菌株,经平板对峙培养初筛和发酵过滤液抑菌试验,得到一个较好的菌株D35,其发酵液经5倍稀释对稻瘟病菌、柑橘炭疽病菌和烟草黑胫病菌的菌丝生长抑制率分别为47.6%、80.3%和54.2%.经培养性状观察,生理生化测定,并结合16S rDNA序列分析,初步鉴定D35为三泽链霉菌(Streptomyces misawanensis).
|
| 关 键 词： | 放线菌 植物病原真菌 抑菌活性 发酵液 |
Isolation,identification and anti-fungal activity of an actinomycete strain D35 |
| |
| ZHU Hong-jian,ZHOU Qian,GAO Bi-da. Isolation,identification and anti-fungal activity of an actinomycete strain D35[J]. Journal of Hunan Agricultural University, 2009, 35(2): 138-141 |
| |
| Authors: | ZHU Hong-jian ZHOU Qian GAO Bi-da |
| |
| Affiliation: | College of Bio-Safety Science and Technology;HNAU;Changsha 410128;China |
| |
| Abstract: | Actinomycetes strain,isolated from soil samples of Hupingshan reservation region in Hunan province by medium with Gause 1,and determined its initial antagonism for plant pathogens through flat confrontation culture.In further bacteriostatic trials by fermentation filtrate,D35 showed significant antifungal activity,with 47.6%,80.3% and 54.2% of mycelium radial growth inhibition of Pyricularia oryzae,Colletotrichum gloeosporioides,Phytophthora parasitica var.nicotianae,respectively,by 5 times culture filtrate... |
| |
| Keywords: | actinomycetes plant pathogens antimicrobial activity fermentation solution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
