| 香蕉转基因受体系统的建立 |
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| 引用本文： | 张妙霞,赖钟雄,何水林,黄玉吉,林玉玲,吴金寿.香蕉转基因受体系统的建立[J].福建农林大学学报(自然科学版),2006,35(5):509-514. |
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| 作者姓名： | 张妙霞 赖钟雄 何水林 黄玉吉 林玉玲 吴金寿 |
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| 作者单位： | 1. 福建农林大学园艺植物生物工程研究所,福建,福州,350002;泉州师范学院图书馆,福建,泉州,362000
2. 福建农林大学园艺植物生物工程研究所,福建,福州,350002 |
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| 摘 要： | 采用横切薄片培养方法建立了香蕉基因转化的适宜受体系统.结果表明:在30℃、黑暗培养15 d,而后转入24℃、光照培养5 d的条件下,香蕉横切薄片的出芽率较高,植株生长较健壮,适宜用作转基因的受体;羧苄青霉素对香蕉薄片的生长没有明显的抑制作用,可以作为转化的抑菌剂;香蕉对潮霉素比较敏感,能够显著抑制香蕉横切薄片的出芽率,确定用40mg.L-1作为潮霉素的筛选含量.
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| 关 键 词： | 香蕉 转基因受体系统 薄片培养 |
| 文章编号： | 1671-5470(2006)05-0509-06 |
| 收稿时间： | 2005-10-23 |
| 修稿时间： | 2006-03-06 |
Development of a transgenic acceptor system for banana |
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| ZHANG Miao-xia,LAI Zhong-xiong,HE Shui-lin,HUANG Yu-ji,LIN Yu-ling,WU Jin-shou.Development of a transgenic acceptor system for banana[J].Journal of Fujian Agricultural and Forestry University,2006,35(5):509-514. |
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| Authors: | ZHANG Miao-xia LAI Zhong-xiong HE Shui-lin HUANG Yu-ji LIN Yu-ling WU Jin-shou |
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| Abstract: | An appropriate transgenic acceptor system for banana(Musa spp.) was developed in this experiment.The high efficiency of shoot differentiation was obtained from the thin-cross-section cultured at 24 ℃ in light for 5 days after being cultured at 30 ℃ in darkness for 15 days.The regenerated plantlets grew well and the thin-cross-sections of the plantlets could be used as transgenic acceptors for Agrobacteriummediated transformation.Carbenicillin could not inhibit growth and differentiation of the thin-crosssections of banana and could be used to kill Agrobacterium during the selection stage.Banana was sensitive to hygromycin B,and hygromycin B could inhibit growth and differentiation of the thincross-sections of banana,which could be used as a selection agent for banana transformation.The results showed that 40 mg·L~(-1) hygromycin was the lowest concentration for the selection. |
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| Keywords: | banana transgenic acceptor system thin-cross-section culture |
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