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| PEX法提取植物组织DNA | |
| 引用本文： | 雷海英,孙毅,仪治本,段永红,王志军. PEX法提取植物组织DNA[J]. 广东农业科学, 2010, 37(5): 178-180 |
| 作者姓名： | 雷海英 孙毅 仪治本 段永红 王志军 |
| 作者单位： | 1. 长治学院生物科学与技术系,山西,长治,046011 2. 山西省农业生物技术研究中心,山西,太原,030031 3. 中北大学,山西,太原,030051 |
| 基金项目： | 山西省高校科技开发项目，山西省长治学院校基金资助项目 |
| 摘 要： | 介绍了一种简单而实用的DNA提取法,利用PEX试剂从高粱与转基因玉米的新鲜叶片中提取植物组织DNA。所得DNA经定量检测后,可满足AFLP分析及PCR-Southern杂交等要求。 |
| 关 键 词： | DNA提取 PCR-Southern杂交 |
PEX method of isolation plant DNA |
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| LEI Hai-ying,SUN YI,YI Zhi-ben,DUAN Yong-hong,WANG Zhi-jun. PEX method of isolation plant DNA[J]. Guangdong Agricultural Sciences, 2010, 37(5): 178-180 | |
| Authors: | LEI Hai-ying SUN YI YI Zhi-ben DUAN Yong-hong WANG Zhi-jun |
| Affiliation: | LEI Hai-ying1,SUN YI2,YI Zhi-ben3,DUAN Yong-hong2,WANG Zhi-jun1 (1.Department of Biological sciences and Biotechnology,Changzhi University,Changzhi,046011,China,2.Agricultural Bio-techonology Research Center of Shanxi Province,Taiyuan 030031,China 3.Department of Environment Engineering,North University of China,Taiyuan 030051,China) |
| Abstract: | A simple and applied DNA extraction method was introduced. The reagent of PEX (potassium ethyl xanthate) was employed to isolate the DNA of the fresh leaves of sorghum and transgenic maize in the method. The DNA collected was quantified and could be applied on AFLP analysis and the PCR-Southern blot hybridization analysis. |
| Keywords: | PEX AFLP |
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