| 桑属植物ITS序列研究与系统发育分析 |
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| 引用本文： | 赵卫国,潘一乐,张志芳. 桑属植物ITS序列研究与系统发育分析[J]. 蚕业科学, 2004, 30(1): 11-14 |
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| 作者姓名： | 赵卫国 潘一乐 张志芳 |
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| 作者单位： | 中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江,212018;中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江,212018;中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江,212018 |
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| 基金项目： | 科技部"攀登计划" , 华东船舶工业学院校科研和教改项目 |
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| 摘 要： | 用PCR产物直接测序法对桑属的 9个种 3个变种共 13份桑种质和构属构树的ITS序列进行了测定。结果表明 :桑属植物ITS1长度平均约为 189bp;桑属 5 8SrRNA为 15 2bp ;ITS2长度平均为 2 12bp ;桑属植物ITS序列G +C含量为 6 0 %左右。用DNASTAR软件构建了桑属植物ITS序列的系统发育树 ,并探讨了参试桑种质的亲缘关系。
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| 关 键 词： | 桑 内转录间隔区(ITS)序列 系统发育 |
| 文章编号： | 0257-4799(2004)01-0011-04 |
| 修稿时间： | 2003-10-30 |
Phylogenetic Relationship of Genus Morus by ITS Sequence Data |
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| ZHAO Weiguo PAN Yile ZHANG Zhifang. Phylogenetic Relationship of Genus Morus by ITS Sequence Data[J]. Acta Sericologica Sinica, 2004, 30(1): 11-14 |
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| Authors: | ZHAO Weiguo PAN Yile ZHANG Zhifang |
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| Abstract: | ITS (internal transcribed spacer) sequences of 13 mulberry materials, which belong to 9 species and 3 varieties, and 1 B. papyrifera were determined using direct sequencing of PCR product in this paper. The length of ITS1 and ITS2 of mulberry is about 189 bp and 211 bp, respectively, while that of 5.8S rRNA is 152 bp. Its G+C contents is about 60%. Phylogenetic analysis of ITS sequences using DNASTAR software indicated that genus Morus is monophyletic group and their affinity relationship is discussed. |
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| Keywords: | Morus albaITSPhylogeny |
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