不同来源日粮铜水平对肉鸡肝线粒体H_2O_2 生成变化的研究

本研究旨在探讨不同来源日粮铜水平对肉鸡肝线粒体H_2O_2生成变化的影响.选用硫酸铜、蛋氨酸铜作为源铜,每种源铜均设4个浓度梯度.288只1日龄科宝商品代肉鸡随机分为8组,每组36只,分别喂以对照日粮(Cu 11 mg·kg~(-1),Ⅰ组)和高铜日粮(Cu 110 mg·kg~(-1),Ⅱ组;Cu 220 mg·kg~(-1),Ⅲ组;Cu 330 mg·kg~(-1),Ⅳ组)60 d,并于12、36和60日龄取样,观察肉鸡肝线粒体H_2O_2生成速率变化.结果:与对照组相比,2种来源的高铜Ⅱ组、Ⅲ组和Ⅳ组肉鸡肝线粒体H_2O_2生成速率显著加快(P＜0.05或P＜0.01);试验第36和60天时,蛋氨酸铜各组肉鸡肝线粒体过氧化氢生成速率均高于相应的硫酸铜各组(P＜0.05或P＜0.01),并且,高铜日粮饲喂的肉鸡肝线粒体H_2O_2生成部位主要集中在线粒体呼吸链复合体Ⅳ.结果表明:高铜日粮可诱导肝脏的氧化应激损伤;同剂量的有机铜螯合物比无机铜更易加快肝脏线粒体H_2O_2生成速率,并可推测,线粒体呼吸链复合体Ⅳ是机体在高铜刺激下攻击的靶部位之一.

Effects of Dietary Copper of Different Sources and Dosages on Hydrogen Peroxide Generation of Mitochondria from Broiler Hepatocyte

The experiment was conducted with the objective of examining the effects of dietary copper of different forms and dosages on hydrogen peroxide (H_2O_2) generation of mitochondria from broiler hepatocyte. Copper sulfate and copper methionine have been used as the copper source, and four concentration copper groups were set for each copper source. 288 broilers (Cobb 500, Gallus domesticus) aged 1 day were randomly divided into 8 groups with 36 each and fed with diets as follows: Controls (Cu 11 mg · kg~(-1) , group Ⅰ ), and high copper (Cu 110 mg · kg~(-1),group Ⅱ ; Cu 220 mg·kg~(-1), high copper group Ⅲ ; Cu 330 mg ·kg~(-1) , high copper group Ⅳ) for 60 days under normal condition, three sample-collections were processed at 12-day-old, 36-dayold and 60-day-old respectively to investigate the changes of H_2O_2 generation of mitochondria from broiler hepatocyte. In result, compared with those of control group, H_2O_2 generation of mitochondria in high copper group Ⅱ , Ⅲ and Ⅳ of two copper sources were increased (P＜0.05 or P＜0.01); At the experimental time of the 36th and 60th day, H_2O_2 generation of mitochondria from broiler hepatocyte in each group of copper methionine were increased compared to those of each group of copper sulfate (P＜0.05 or P＜0.01). The location of H_2O_2 generation of mitochondria from broiler fed with high dietary copper were mainly concentrated in mitochondrial complex Ⅳ. The results obtained above indicated that high dietary copper could induce oxidized stress damage in liver; at the same level of dietary copper supplement, the organic copper will lead to much faster H_2O_2 generation velocity of mitochondria from broiler hepatocyte than inorganic copper. The results can also suggest that mitochondrial complex Ⅳ might be one of attack target spots under high dietary copper stimulation of organism.